Mycobiota in the Carposphere of Sour and Sweet Cherries and Antagonistic Features of Potential Biocontrol Yeasts

Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from privat...

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Bibliographic Details
Published in:Microorganisms (Basel) 2021-06, Vol.9 (7), p.1423
Main Authors: Stanevičienė, Ramunė, Lukša, Juliana, Strazdaitė-Žielienė, Živilė, Ravoitytė, Bazilė, Losinska-Sičiūnienė, Regina, Mozūraitis, Raimondas, Servienė, Elena
Format: Article
Language:English
Subjects:
antagonistic activity
Berries
Biological control
Cherries
cherry
Deoxyribonucleic acid
Disease control
DNA
Economic importance
Food
Food industry
Fruits
fungal communities
Fungi
Laboratories
Metabolites
Microorganisms
P. avium
P. cerasus
Pathogens
Plantations
Prunus avium
Prunus cerasus
rRNA
Sequence analysis
Yeast
Yeasts
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Summary:Sour cherries (Prunus cerasus L.) and sweet cherries (P. avium L.) are economically important fruits with high potential in the food industry and medicine. In this study, we analyzed fungal communities associated with the carposphere of sour and sweet cherries that were freshly harvested from private plantations and purchased in a food store. Following DNA isolation, a DNA fragment of the ITS2 rRNA gene region of each sample was individually amplified and subjected to high-throughput NGS sequencing. Analysis of 168,933 high-quality reads showed the presence of 690 fungal taxa. Investigation of microbial ASVs diversity revealed plant-dependent and postharvest handling-affected fungal assemblages. Among the microorganisms inhabiting tested berries, potentially beneficial or pathogenic fungi were documented. Numerous cultivable yeasts were isolated from the surface of tested berries and characterized by their antagonistic activity. Some of the isolates, identified as Aureobasidium pullulans, Metschnikowia fructicola, and M. pulcherrima, displayed pronounced activity against potential fungal pathogens and showed attractiveness for disease control.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9071423