Rapid detection of respiratory organisms with the FilmArray respiratory panel in a large children's hospital in China

Respiratory tract infections (RTIs) are the most common illness in children, and rapid diagnosis is required for the optimal management of RTIs, especially severe infections. Nasopharyngeal swab or sputum specimens were collected from children aged 19 days to 15 years who were admitted to a hospital...

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Bibliographic Details
Published in:BMC infectious diseases 2018-10, Vol.18 (1), p.510-510, Article 510
Main Authors: Li, Jin, Tao, Yue, Tang, Mingyu, Du, Bailu, Xia, Yijun, Mo, Xi, Cao, Qing
Format: Article
Language:English
Subjects:
Adenoviruses
Automation
Children
FilmArray respiratory panel
Hospitals
Immunology
Infections
Infectious diseases
Influenza
Influenza A
Organisms
Parainfluenza
Pathogens
Pediatrics
Pertussis
Pneumonia
Polymerase chain reaction
Respiratory organisms
Respiratory syncytial virus
Respiratory tract
Respiratory tract diseases
Respiratory tract infections
Rhinovirus
Seasonal variations
Sputum
Viruses
Whooping cough
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Summary:Respiratory tract infections (RTIs) are the most common illness in children, and rapid diagnosis is required for the optimal management of RTIs, especially severe infections. Nasopharyngeal swab or sputum specimens were collected from children aged 19 days to 15 years who were admitted to a hospital in Shanghai and diagnosed with RTIs. The specimens were tested with the FilmArray Respiratory Panel, a multiplex PCR assay that detects 16 viruses, Mycoplasma pneumoniae (M. pneumoniae), Bordetella pertussis (B. pertussis) and Chlamydophila pneumoniae (C. pneumoniae). Among the 775 children studied, 626 (80.8%, 626/775) tested positive for at least one organism, and multiple organisms were detected in 198 (25.5%). Rhinoviruses/enteroviruses (25.5%, 198/775) were detected most often, followed by respiratory syncytial virus (19.5%, 151/775), parainfluenza virus 3 (14.8%, 115/775), influenza A or B (10.9%), adenovirus (10.8%), M. pneumoniae (10.6%) and B. pertussis (6.3%). The prevalence of organisms differed by age, and most of the viruses were more common in winter. Of the 140 children suspected of having pertussis, 35.0% (49/140) tested positive for B. pertussis. FilmArray RP allows the rapid simultaneous detection of a wide number of respiratory organisms, with limited hands-on time, in Chinese pediatric patients with RTIs.
ISSN:1471-2334
1471-2334
DOI:10.1186/s12879-018-3429-6