Biochemical Characterization and Structural Modeling of Fused Glucose-6-Phosphate Dehydrogenase-Phosphogluconolactonase from Giardia lamblia

• Published in: International journal of molecular sciences 2018-08, Vol.19 (9), p.2518
• Main Authors: Morales-Luna, Laura, Serrano-Posada, Hugo, González-Valdez, Abigail, Ortega-Cuellar, Daniel, Vanoye-Carlo, America, Hernández-Ochoa, Beatriz, Sierra-Palacios, Edgar, Rufino-González, Yadira, Castillo-Rodríguez, Rosa Angélica, Pérez de la Cruz, Verónica, Moreno-Vargas, Liliana, Prada-Gracia, Diego, Marcial-Quino, Jaime, Gómez-Manzo, Saúl
• Format: Article
• Language: English
• Subjects: 6-Phosphogluconolactonase; Amino Acid Sequence; Amino acids; Base Sequence; Bioinformatics; bioinformatics analysis; Carboxylic Ester Hydrolases - chemistry; Carboxylic Ester Hydrolases - genetics; Carboxylic Ester Hydrolases - metabolism; Cell cycle; Chromosome 6; Cloning; Dehydrogenases; DNA, Complementary - chemistry; DNA, Complementary - genetics; Drug development; Enzyme Activation; Enzyme Stability; Enzymes; Fatty acids; G6PD; Gene Expression; Genes; Giardia lamblia; Giardia lamblia - enzymology; Giardia lamblia - genetics; Glucose 6 phosphate dehydrogenase; Glucosephosphate dehydrogenase; Glucosephosphate Dehydrogenase - chemistry; Glucosephosphate Dehydrogenase - genetics; Glucosephosphate Dehydrogenase - metabolism; Guanidine hydrochloride; Humans; Hydrogen-Ion Concentration; Kinetics; Metabolism; Modelling; Models, Molecular; Pentose; Pentose phosphate pathway; Pharmacology; Protein Conformation; Protein structure; Protein turnover; Proteins; purification; recombinant expression; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Secondary structure; Structural analysis; Structural stability; Structure-Activity Relationship; Structure-function relationships; Temperature; Tertiary structure; Thermal stability; three-dimensional structure; Trophozoites
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19092518

Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme in the pentose phosphate pathway and is highly relevant in the metabolism of Previous reports suggested that the G6PD gene is fused with the 6-phosphogluconolactonase (6PGL) gene ( ). Therefore, in this work, we decided to characterize the fused G6PD-6PGL protein in First, the gene of fused with the gene ( :: ) was isolated from trophozoites of and the corresponding G6PD::6PGL protein was overexpressed and purified in . Then, we characterized the native oligomeric state of the G6PD::6PGL protein in solution and we found a catalytic dimer with an optimum pH of 8.75. Furthermore, we determined the steady-state kinetic parameters for the G6PD domain and measured the thermal stability of the protein in both the presence and absence of guanidine hydrochloride (Gdn-HCl) and observed that the G6PD::6PGL protein showed alterations in the stability, secondary structure, and tertiary structure in the presence of Gdn-HCl. Finally, computer modeling studies revealed unique structural and functional features, which clearly established the differences between G6PD::6PGL protein from and the human G6PD enzyme, proving that the model can be used for the design of new drugs with antigiardiasic activity. These results broaden the perspective for future studies of the function of the protein and its effect on the metabolism of this parasite as a potential pharmacological target.