Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification

Plant peroxidases (PODs) are involved in diverse physiological processes, including defense against pathogens and insects. Contrary to their biological importance, only very few plant PODs have been proven on protein level, because their low abundance makes them difficult to detect in standard prote...

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Published in:Frontiers in plant science 2015-08, Vol.6, p.670-670
Main Authors: López-Castillo, Laura M, López-Arciniega, Janet A I, Guerrero-Rangel, Armando, Valdés-Rodríguez, Silvia, Brieba, Luis G, García-Lara, Silverio, Winkler, Robert
Format: Article
Language:English
Subjects:
insect resistance
Maize (Zea mays)
Maize kernel
Peroxidase
plant breeding
plant proteomics
Plant Science
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Summary:Plant peroxidases (PODs) are involved in diverse physiological processes, including defense against pathogens and insects. Contrary to their biological importance, only very few plant PODs have been proven on protein level, because their low abundance makes them difficult to detect in standard proteomics work-flows. A statistically significant positive correlation between POD activity and post-harvest insect resistance has been found for maize (Zea mays, p84C3) kernels. In combining activity-directed protein purification, genomic and proteomic tools we found that protein B6T173 (ZmPrx35) is responsible for the majority of the POD activity of the kernel. We successfully produced recombinant ZmPrx35 protein in Escherichia coli and demonstrate both, in vitro activity and the presence of a haem (heme) cofactor of the enzyme. Our findings support the screening for insect resistant maize variants and the construction of genetically optimized maize plants.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2015.00670