Role of MicroRNAs in the Regulation of Subcutaneous White Adipose Tissue in Individuals With Obesity and Without Type 2 Diabetes

Obesity is a high-risk factor for such comorbidities as cardiovascular disease, several types of cancer, and type 2 diabetes; however not all individuals with obesity have such complications. Approximately 20% of individuals with obesity are metabolically healthy. This study focused on differences b...

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Published in:Frontiers in endocrinology (Lausanne) 2019-12, Vol.10, p.840-840
Main Authors: Brovkina, O, Nikitin, A, Khodyrev, D, Shestakova, E, Sklyanik, I, Panevina, A, Stafeev, Iurii, Menshikov, M, Kobelyatskaya, A, Yurasov, A, Fedenko, V, Yashkov, Yu, Shestakova, M
Format: Article
Language:English
Subjects:
Endocrinology
metabolically healthy obesity
metalloproteinases
microRNA
RNAseq
RUNX2
SMAD4
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Summary:Obesity is a high-risk factor for such comorbidities as cardiovascular disease, several types of cancer, and type 2 diabetes; however not all individuals with obesity have such complications. Approximately 20% of individuals with obesity are metabolically healthy. This study focused on differences between obese individuals with and without type 2 diabetes (T2D+ and T2D-, respectively) on the transcriptome level. Subjects included were 35 T2D- patients with obesity and 35 T2D+ patients with obesity with the same body mass index (BMI). The study was based on the transcription analysis of mRNA and microRNAs (miRs) by RNAseq. In the first step, we performed RNAseq of miRs, in the second step, we analyzed only those mRNA, which appeared targets for significant miRs from the first step. All RNAseq results were validated by qPCR. There were seven miRs differently expressed with adjusted -value
ISSN:1664-2392
1664-2392
DOI:10.3389/fendo.2019.00840