MEASUREMENT OF THE ANTIBODY RESPONSE BY THE ELIMINATION OF I 131-LABELED PROTEINS. II. THE ELIMINATION OF I 131-HORSE SERUM ALBUMIN FROM THE BLOOD OF IMMUNIZED LAF 1 MICE

The elimination of I 131-labeled horse serum albumin was used as a semi-quantitative measure for antibody production in mice. The parameters used for measuring the antibody production by means of the antigen elimination method were the induction period, the slope of the elimination curve and the T99...

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Bibliographic Details
Main Authors: Weyzen,Walter W H, Vattuone,Gerald M, Silverman,Myron S
Format: Report
Language:English
Subjects:
ANTIBODIES
ANTIGEN ANTIBODY REACTIONS
ANTIGENS
BLOOD PROTEINS
EXCRETION
IMMUNE SERUMS
IMMUNITY
LABELED SUBSTANCES
Medicine and Medical Research
RADIATION EFFECTS
RADIOACTIVE ISOTOPES
Radiobiology
RESPONSE(BIOLOGY)
TRACER STUDIES
WHOLE BODY IRRADIATION
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Summary:The elimination of I 131-labeled horse serum albumin was used as a semi-quantitative measure for antibody production in mice. The parameters used for measuring the antibody production by means of the antigen elimination method were the induction period, the slope of the elimination curve and the T99 (time, in hours, required for the elimination of 99% of the amount of antigen present in the blood at time zero). It was found that the quantitative measurement of the immune elimination depended on the immunizing dose and on the tracer dose. The effects of various immunizing doses and of tracer doses on the observed immune response could be correlated in part with the amount of antigen present in the plasma at the onset of antibody formation. It was observed that only a fraction of the I 131-labeled alum-precipitated horse serum albumin injected was able to equilibrate in the intra- and extravascular spaces. The antigen in this 'albumin pool' was eliminated in a manner identical to intravenously injected I 131-horse serum albumin in non-immunized mice. The remaining portion of the antigen, presumably localized in various tissues, was eliminated at a much slower rate. It was postulated that the stored antigen was able to react with antibodies in the circulation, thus decreasing the amount of antibody available for elimination of labeled antigen. The possible implication of these findings for measurement of antibody formation under standard conditions is discussed. (Author)