Nutrient (Lipid, Nucleoside, and Soluble Metabolite) Studies on Intraerythrocytic Malaria Parasites

A lipid emulsification technique for use in perfused parallel suspension cultures of intraerythrocytic malaria was developed. Supplementation of commercial tissue culture media with cholesterol, lecithin, saffower oil and corn oil yielded no indication as to the missing red cell survival and malaria...

Full description

Saved in:
Bibliographic Details
Main Author: Tiner,Jack D
Format: Report
Language:English
Subjects:
ANTIMALARIALS
BLOOD SERUM
CULTURE MEDIA
GROWTH(GENERAL)
IN VITRO ANALYSIS
MALARIA
Medicine and Medical Research
NUCLEOSIDES
NUTRITION
PARASITIC DISEASES
PLASMODIUM
PLASMODIUM BERGHEI
SILICON
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A lipid emulsification technique for use in perfused parallel suspension cultures of intraerythrocytic malaria was developed. Supplementation of commercial tissue culture media with cholesterol, lecithin, saffower oil and corn oil yielded no indication as to the missing red cell survival and malaria growth factor(s). Four antimarial drugs were used to test the perfusion system, and they interacted with segments of silicone rubber tubing that pass through the peristaltic pump. Quinine and chloroquine each are absorbed by that tubing when these are perfused as 50 ppm solutions. There is enough residue activity to continue poisoning cultures in the second subsequent experiment in which it is used to deliver untreated medium. Other pieces of silicone tubing were exposed to 100 ppm quinacrine and pyrimethamine solutions and then active residues were released into untreated medium that was delivered through the same pieces in the next experiment. These two antimalarials diffused through the wall of the 100 ppm treated tubes and into adjacent non-medicated control tubes in the peristaltic pump. In two immediately following experiments these control tubes delivered inhibitory media. Distribution of a yellow, ultraviolet fluorescent stain in the silicone rubber provided evidence that the inhibitions were drug-caused in the case of quinacrine. Tests with various lengths of silicone tubing reaffirmed that it can be used for studies that do not involve antimalarials. (Author)