Analysis of Investigational Drugs in Biological Fluids Method Development and Routine Assay

The purpose of work under this contract is to develop and routinely use analytical methods for the determination of the concentration in biological specimens of investigational drugs in support of pharmacokinetic and bioavailability studies undertaken for the purpose of new drug development for the...

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Bibliographic Details
Main Authors: Lin, Emil T, Gee, Winnie L
Format: Report
Language:English
Subjects:
ANTIMALARIALS
ASSAYING
BIOLOGY
BLOOD
BLOOD PLASMA
CARTRIDGES
CHOLINESTERASE INHIBITORS
DOGS
DRUGS
ELUTION
FLUIDS
HUMANS
MILITARY FORCES(UNITED STATES)
PHARMACOKINETICS
Pharmacology
PHYSOSTIGMINE
PLASMAS(PHYSICS)
PRECIPITATION
PROTEINS
RADIOPROTECTIVE AGENTS
SAMPLING
URINE
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Summary:The purpose of work under this contract is to develop and routinely use analytical methods for the determination of the concentration in biological specimens of investigational drugs in support of pharmacokinetic and bioavailability studies undertaken for the purpose of new drug development for the US military establishment. Accepted scientific procedures including normal and reversed phase high-performance liquid chromatographic methods, post column derivatization, and protein precipitation and cartridge elution sample clean up procedures were employed in development and routine work. Methodologies for six drugs (for the assay of samples for WR 2721 in plasma, WR 3689 in plasma, pyridostigmine in urine, mefloquine in plasma, physostigmine in plasma and WR6026 in blood) were under development, two routine analyses (for determination of the concentrations of mefloquine in plasma and pyridostigmine in plasma and urine) have been performed in support of bioavailability and pharmacokinetics studies, and routine analyses (for pyridostigmine in dog plasma and pyridostigmine in human plasma) are in progress in support of other studies. Significant progress has been made in meeting the objectives of the contract.