Scaling of Microfluidic Biodevices

(1)We will first determine the physical limits to speed and size scaling of established biochemical methods for identification of DNA, proteins, and cells. As necessary, we will optimize methods and materials. The object is to establish scaling of the working biochemistry in order to better design d...

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Bibliographic Details
Main Author: Ehrlich, Daniel J
Format: Report
Language:English
Subjects:
APP(ARMED FORCES INSTITUTE OF PATHOLOGY)
ARMY EQUIPMENT
ARMY RESEARCH
BIOASSAY
BIOCHEMISTRY
COSTS
DATA ACQUISITION
DEOXYRIBONUCLEIC ACIDS
Fluidics and Fluerics
Genetic Engineering and Molecular Biology
INFECTIOUS DISEASES
INTEGRATED OPTICS
INTEGRATED SYSTEMS
MICROFLUIDIC BIODEVICES
MICROMINIATURIZATION
MINIATURIZATION
SCALING FACTOR
USAMRIID(US ARMY RESEARCH INSTITUTE FOR INFECTIOUS DISEASES)
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Summary:(1)We will first determine the physical limits to speed and size scaling of established biochemical methods for identification of DNA, proteins, and cells. As necessary, we will optimize methods and materials. The object is to establish scaling of the working biochemistry in order to better design devices (below). For the most part, cost and speed will both scale very favorably with miniaturization, however, the materials and detailed chemical mechanism will require optimizations to obtain the potential advantages of integrated devices. Integrated devices also present new problems to biochemical analysis, for example, stemming from high surface-to-volume ratio. The working mechanism of new devices is certain to remain biochemical in nature, but must be optimized for the application. We propose to gather this necessary information on scaling behavior and to confirm new optimizations using microfabricated flow apparatus in combination with conventional apparatus available at the Whitehead Institute. (2)We will then apply the results above to fabricate and test system modules for high-speed identification and quantitative assay of biochemical materials. The main focus will be on optimizing modules for assay speed, with an emphasis on all contributions to the time budget from sample injection to data collection. Appropriate microfabrication and integrated optics technology will be used. The object is to confirm scaling in analysis time and sample size in highly functional devices. Qualification of the new devices for fast forensic and biochemical assay applications will be made at the DoD application centers (specifically at the Armed Forces Institute of Pathology (APP) The US Army Materials Research Institute for Infectious Diseases (USAMRIID), and at the Aberdeen Proving Grounds (ERDEC).