Cynomolgus Macaque as an Animal Model for Severe Acute Respiratory Syndrome

The emergence of severe acute respiratory syndrome (SARS) in 2002 - 2003 had a tremendous global impact. Adequate animal models are required to study the underlying pathogenesis of SARS-associated coronavirus (SARS-CoV) infection and to develop effective vaccines and therapeutics. In order to charac...

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Bibliographic Details
Main Authors: Lawler, James V, Endy, Timothy P, Hensley, Lisa E, Garrison, Aura, Fritz, Elizabeth A, Lesar, May, Baric, Ralph S, Kulesh, David A, Norwood, David A, Wasieloski, Leonard P
Format: Report
Language:English
Subjects:
Anatomy and Physiology
ANTIBODIES
Biology
BRONCHI
CONJUNCTIVITIS
CORONAVIRUS
INFECTIOUS DISEASES
INOCULATION
MACAQUE MONKEYS
NONHUMAN PRIMATES
PNEUMONIA
RADIOGRAPHY
RESPIRATORY SYSTEM
SARS(SEVERE ACUTE RESPIRATORY SYNDROMES)
SARS-COV(SARS-ASSOCIATED CORONAVIRUS)
VACCINES
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Summary:The emergence of severe acute respiratory syndrome (SARS) in 2002 - 2003 had a tremendous global impact. Adequate animal models are required to study the underlying pathogenesis of SARS-associated coronavirus (SARS-CoV) infection and to develop effective vaccines and therapeutics. In order to characterize clinically relevant parameters of SARS-CoV infection in non-human primates, we infected cynomolgus macaques with SARS-CoV in three groups: Group I was infected in the nares and bronchus, group II in the nares and conjunctiva and Group II intravenously. Animals in Groups I and II developed mild-moderate symptomatic illness. All animals demonstrated evidence of viral replication and developed neutralizing antibodies. Chest radiographs from several animals in Groups I and II revealed unifocal or multifocal pneumonia that peaked between days 8 -10 postinfection. Clinical laboratory tests were not significantly changed. Overall, inoculation by a mucosal route produced more significant disease that intravenous inoculation. SARS-CoV infection of cynomolgus macaques did not reproduce the severe illness seen in the majority of human cases of SARS; however, our results suggest similarities to the more mild syndrome of SARS infection characteristically seen in young children. The original document contains color images.