Deactivating Chemical Agents Using Enzyme-Coated Nanofibers Formed by Electrospinning

The coaxial electrospinning technique was investigated as a novel method to create stabilized, enzyme-containing fibers that have the potential to provide enhanced protection from chemical agents. Electrospinning is a versatile technique for the fabrication of polymer fibers with large length (cm to...

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Bibliographic Details
Main Authors: Han, Daewoo, Kirby, Romy, Filocamo, Shaun F, Steckl, Andrew
Format: Report
Language:English
Subjects:
CHEMICAL AGENTS
Chemical, Biological and Radiological Warfare
COATINGS
COAXIAL ELECTROSPINNING
DECONTAMINATION
DFPASE
ENZYMES
FIBERS
MATS
NANOFIBERS
NANOSTRUCTURES
NEUTRALIZATION
Polymer Chemistry
POLYMERS
SPINNING(INDUSTRIAL PROCESSES)
Textiles
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Summary:The coaxial electrospinning technique was investigated as a novel method to create stabilized, enzyme-containing fibers that have the potential to provide enhanced protection from chemical agents. Electrospinning is a versatile technique for the fabrication of polymer fibers with large length (cm to km): diameter (nm to um) aspect ratios. The large surface to volume ratios, along with the biofriendly nature of this technique, enables the fabrication of fiber mats with high enzyme concentrations, which amplify the catalytic activity per unit volume of membrane. Blended composite (single-source) fibers incorporate enzyme throughout the fiber, which may limit substrate accessibility to the enzyme. In contrast, core/sheath fibers can be produced by coaxial electrospinning with very high enzyme loading (80%) in the sheath without noticeable loss of enzymatic activity. Several core-sheath combinations have been explored with the toxin-mitigating enzyme DFPase in order to achieve fibers with optimum properties. The concentration of fluoride released, normalized for the amount of protein incorporated into the sheath, was used as a measure of the enzyme activity versus time. The coaxial core/sheath combination of PEO and DFPase produced the highest activity (7.3 mM/mg). Prepared in collaboration with University of Cincinnati, Nanoelectronics Laboratory, Cincinnati, OH. Reprint of article published in the peer-reviewed journal ACS Applied Materials & Interfaces: 2011 December; 3(12):4633-9. doi: 10.1021/am201064b. The original document contains color images.