Functional Deficits and Insulin-Like Growth Factor-I Gene Expression Following Tourniquet-Induced Injury of Skeletal Muscle in Young and Old Rats

This study investigated the effect of age on recovery of skeletal muscle from an ischemia-reperfusion (I/R)-induced injury. Young (6 mo old) and old (24 27 mo old) Sprague-Dawley rats underwent a 2-h bout of hindlimb ischemia induced by a pneumatic tourniquet (TK). The TK was released to allow reper...

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Bibliographic Details
Main Authors: Hammers, David W, Merritt, Edward K, Matheny, Wayne, Adamo, Martin L, Walters, Thomas J, Estep, J S, Farrar, Roger P
Format: Report
Language:English
Subjects:
AGING(PHYSIOLOGY)
Anatomy and Physiology
CELLS(BIOLOGY)
CLINICAL MEDICINE
DAMAGE CONTROL
DRESSINGS
EXTREMITIES
GENE EXPRESSION
Genetic Engineering and Molecular Biology
HEALING
HISTOLOGY
INSULIN
INSULIN-LIKE GROWTH FACTOR-I
IR(ISCHEMIA-REPERFUSION)
ISCHEMIA
MEDICAL EQUIPMENT
Medical Facilities, Equipment and Supplies
Medicine and Medical Research
MUSCLE REGENERATION
MUSCLES
MUSCULOSKELETAL SYSTEM
PATHOLOGY
PERFUSION
PHYSIOLOGICAL EFFECTS
RATS
RECOVERY
REGENERATION(ENGINEERING)
REGENERATION(PHYSIOLOGY)
RESPONSE(BIOLOGY)
RIBONUCLEIC ACIDS
SACROPENIA
TK(TOURNIQUETS)
WOUNDS AND INJURIES
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Summary:This study investigated the effect of age on recovery of skeletal muscle from an ischemia-reperfusion (I/R)-induced injury. Young (6 mo old) and old (24 27 mo old) Sprague-Dawley rats underwent a 2-h bout of hindlimb ischemia induced by a pneumatic tourniquet (TK). The TK was released to allow reperfusion of the affected limb, and animals were divided into 7- and 14-day recovery groups. Maximum plantar flexor force production was assessed in both 7- and 14-day recovery groups of both ages, followed by histological evaluation. Subsequent analysis of IGF-I gene expression and intracellular signaling in 7-day recovery muscles was performed by RT-PCR and Western blotting, respectively. Old rats had significantly greater deficits in force production and exhibited more evidence of histological pathology than young at both 7 and 14 days postinjury. In addition, old rats demonstrated an attenuated upregulation of IGF-I mRNA and induction of proanabolic signaling compared with young in response to injury. We conclude that aged skeletal muscle exhibits more damage and/or defective regeneration following I/R and identify an age-associated decrease in local IGF-I responsiveness as a potential mechanism for this phenomenon. Published in the Journal of Applied Physiology, v105 p1274-1281, 31 July 2008.