Mapping In Vivo Tumor Oxygenation within Viable Tumor by19 F-MRI and Multispectral Analysis

• Published in: Neoplasia (New York, N.Y.) N.Y.), 2013, Vol.15 (11), p.1241-IN1
• Main Authors: Shi, Yunzhou, Oeh, Jason, Eastham-Anderson, Jeffrey, Yee, Sharon, Finkle, David, Peale, Franklin V, Ross, Jed, Hedehus, Maj, van Bruggen, Nicholas, Venook, Rayna, Ross, Sarajane, Sampath, Deepak, Carano, Richard A.D
• Format: Article
• Language: English
• Subjects: Oncology
• ISSN: 1476-5586
• DOI: 10.1593/neo.131468

Abstract Quantifying oxygenation in viable tumor remains a major obstacle toward a better understanding of the tumor microenvironment and improving treatment strategies. Current techniques are often complicated by tumor heterogeneity. Herein, a novel in vivo approach that combines19 F magnetic resonance imaging (19 F-MRI)R1 mapping with diffusionbased multispectral (MS) analysis is introduced. This approach restricts the partial pressure of oxygen (pO2 ) measurements to viable tumor, the tissue of therapeutic interest. The technique exhibited sufficient sensitivity to detect a breathing gas challenge in a xenograft tumor model, and the hypoxic region measured by MS19 F-MRI was strongly correlated with histologic estimates of hypoxia. This approach was then applied to address the effects of antivascular agents on tumor oxygenation, which is a research question that is still under debate. The technique was used to monitor longitudinal pO2 changes in response to an antibody to vascular endothelial growth factor (B20.4.1.1) and a selective dual phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor (GDC-0980). GDC-0980 reduced viable tumor pO2 during a 3-day treatment period, and a significant reduction was also produced by B20.4.1.1. Overall, this method provides an unprecedented view of viable tumor pO2 and contributes to a greater understanding of the effects of antivascular therapies on the tumor's microenvironment.