Cloning and characterization of a Schizophyllum gene with Abeta6 mating-type activity

The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, Aalpha and Abeta. Aalpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations o...

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Bibliographic Details
Published in:Current genetics 1996, Vol.29 (2)
Main Authors: Shen, G.P, Park, D.C, Ullrich, R.C, Novotny, C.P. (Vermont Univ., Burlington (USA). Dept. of Microbiology and Molecular Genetics)
Format: Article
Language:English
Subjects:
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
FECONDATION
FECUNDACION
GENE
GENES
SCHIZOPHYLLUM COMMUNE
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Summary:The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, Aalpha and Abeta. Aalpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci, Aalpha and Abeta, both manage to regulate the same pathway. Here the structure of a gene with Abeta6 activity is reported. This gene, denoted AbetaV6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in AalphaY1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to AalphaY, AalphaZ, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus while Aalpha and Abeta encode different proteins, the general mechanism by which Aalpha and Abeta components signal A-regulated development may be similar.
ISSN:0172-8083
1432-0983