Detection of canine parvovirus (CPV) DNA by polymerase chain reaction assay and its prevalence in dogs in and around Kolkata, West Bengal

The early detection of the canine parvo virus (CPV) is of paramount importance. Canine faecal samples from 306 dogs suffering from gastroenteritis in and around Kolkata were collected, and DNA was extracted by phenol-chloroform method. CPV vaccine strain was used as a positive control for CPV. Polym...

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Bibliographic Details
Published in:Indian journal of animal sciences 2006-04, Vol.76 (4)
Main Authors: Biswas, S, Das, P.J, Ghosh, S.K, Pradhan, N.R
Format: Article
Language:English
Subjects:
ADN
BENGALA OCCIDENTAL
BENGALE OCCIDENTAL
CANINE PARVOVIRUS
CHIEN
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
DNA
DOGS
http://www.fao.org/aos/agrovoc#c_2238
http://www.fao.org/aos/agrovoc#c_2347
http://www.fao.org/aos/agrovoc#c_2352
http://www.fao.org/aos/agrovoc#c_34079
http://www.fao.org/aos/agrovoc#c_35615
http://www.fao.org/aos/agrovoc#c_8356
PARVOVIRUS CANIN
PARVOVIRUS CANINO
PCR
PERRO
WEST BENGAL
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Summary:The early detection of the canine parvo virus (CPV) is of paramount importance. Canine faecal samples from 306 dogs suffering from gastroenteritis in and around Kolkata were collected, and DNA was extracted by phenol-chloroform method. CPV vaccine strain was used as a positive control for CPV. Polymerase chain reaction (PCR) was carried out to amplify VI1/VP2 gene using a set of 19-mer primers (CPV-P)1 (Forward):5-ATG GCA CCT CCG GCA AAG A-3 CPV-P2 (Reverse): 5-TTT CTA GGT GCT AGT TGA G-3)). A PCR product of approximately 2.2 kb was generated with positive faecal samples and vaccine strain CPV virus After screening, 103 dogs were found positive for CPV, but no sex variation was noted amongst the CPV positive cases. Dogs, of the age group of 0-6 months were mostly susceptible with highest mortality rate followed by 6-12 months and 12 and above months of age and highest occurrence was noted during summer followed by rainy season and winter.
ISSN:0367-8318