Transgenic tobacco plants expressing pea chloroplast Nmdh c DNA in sense and antisense orientation. Effects on NADP-malate dehydrogenase level, stability of transformants, and plant growth

A full-length cDNA encoding light-activated chloroplast NADP-malate dehydrogenase (NADP-MDH) (EC 1.1.1.82) from pea (Pisum sativum L.) was introduced in the sense and antisense orientation into tobacco (Nicotiana tabacum L.). Transgenic plants with decreased or increased expression levels were obtai...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1997-10, Vol.115 (2)
Main Authors: Faske, M, Backhausen, J.E, Sendker, M, Singer-Bayrle, M, Scheibe, R, Schaewen, A. von
Format: Article
Language:English
Subjects:
ACIDE MALIQUE
ACIDO MALICO
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ADN
ALCOHOL DEHYDROGENASE
ALCOHOL DESHIDROGENASA
ALCOHOL OXIDOREDUCTASES
ALCOOL DESHYDROGENASE
ANTISENSE DNA
CHLOROPLASTE
CHLOROPLASTS
CLOROPLASTO
CODE GENETIQUE
CODIGO GENETICO
COENZIMAS
COENZYME
COENZYMES
COMPLEMENTARY DNA
CRECIMIENTO
CROISSANCE
DNA
ENZYMIC ACTIVITY
ETAPAS DE DESARROLLO DE LA PLANTA
EXPRESION GENICA
EXPRESSION DES GENES
FERTILIDAD
FERTILITE
FERTILITY
FEUILLE
GENE
GENE EXPRESSION
GENE TRANSFER
GENES
GENETIC CODE
GENETIC REGULATION
GENETIC VARIATION
GENETICA
GENETICS
GENETIQUE
GENOTIPOS
GENOTYPE
GENOTYPES
GROWTH
HOJAS
LEAVES
MALIC ACID
MEASUREMENT
MEDICION
MESURE
NADH
NICOTIANA TABACUM
NUCLEOTIDE
NUCLEOTIDES
NUCLEOTIDOS
PISUM SATIVUM
PLANT DEVELOPMENTAL STAGES
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
STADE DE DEVELOPPEMENT VEGETAL
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSGENIC PLANTS
VARIACION GENETICA
VARIATION GENETIQUE
WILD STRAINS
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Summary:A full-length cDNA encoding light-activated chloroplast NADP-malate dehydrogenase (NADP-MDH) (EC 1.1.1.82) from pea (Pisum sativum L.) was introduced in the sense and antisense orientation into tobacco (Nicotiana tabacum L.). Transgenic plants with decreased or increased expression levels were obtained. Because of substantial age-dependent differences in individual leaves of a single plant, standardization of NADP-MDH levels was required first. Then, extent and stability of over- or under-expression of Nmdh, the gene encoding NADP-MDH, was characterized in the various transformants. Frequently, cosuppression effects were observed, indicating sufficient homology between the endogenous tobacco and the heterologous pea gene. Analysis of the T1 and T2 progeny of a series of independent transgenic lines revealed that NADP-MDH capacity ranged between 10% and greater than or equal to 10-fold compared with the wild type. Under ambient conditions whole-plant development, growth period, and fertility were unaffected by NADP-MDH reduction to 20% of the wild-type level; below this threshold plant growth was retarded. A positive growth effect was registered in young plants with stably enhanced NADP-MDH levels within a defined developmental window
ISSN:0032-0889
1532-2548