AFLP fingerprinting for paternity testing in ducks

1. The accuracy and reproducibility of AFLP fingerprinting was investigated in the duck (Anas Platyrhynchos), using a multicolour fluorescent labeling technique. The fluorescent labelling fragments were separated on a capillary electrophoresis-base ABI PRISM 3100 Genetic Analyzer. 2. A total of 337...

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Bibliographic Details
Published in:British poultry science 2007-06, Vol.48 (3), p.323-330
Main Authors: Huang, C.W, Cheng, Y.S, Rouvier, R, Yang, K.T, Wu, C.P, Huang, M.C
Format: Article
Language:English
Subjects:
accuracy
amplified fragment length polymorphism
Anas platyrhynchos
Animal Husbandry
Animals
Breeding
DNA fingerprinting
DNA Fingerprinting - methods
DNA Fingerprinting - veterinary
ducks
Ducks - genetics
Fathers
Female
genetic markers
genetic polymorphism
genetic variation
genotype
Life Sciences
Male
males
Nucleic Acid Amplification Techniques
paternity
paternity testing
Quantitative Trait Loci
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Summary:1. The accuracy and reproducibility of AFLP fingerprinting was investigated in the duck (Anas Platyrhynchos), using a multicolour fluorescent labeling technique. The fluorescent labelling fragments were separated on a capillary electrophoresis-base ABI PRISM 3100 Genetic Analyzer. 2. A total of 337 AFLP peaks with 103 of them being polymorphic markers were generated by 16 sets consisting of EcoRI/TaqI primer pair combinations. The number and size range of AFLP polymorphisms detected per primer pair varied from 3 to 11 and 58 to 290 bp, respectively. About 30.6% (103/337) of AFLP peaks were detected polymorphisms, with an average of 6.4 polymorphic markers per primer pair. 3. The clear polymorphic peaks were amplified with EcoR+AC/Taq+AC primer combinations. The AFLP peaks showed high reproducibility. From the family testing, we found that the fingerprints of all the offspring were derived from one or other parent. Therefore, we conclude that AFLP fingerprinting might be a suitable method for duck paternity testing.
ISSN:0007-1668
1466-1799
DOI:10.1080/00071660701370459