Detection of Reactive Oxygen Species via Endogenous Oxidative Pentose Phosphate Cycle Activity in Response to Oxygen Concentration: IMPLICATIONS FOR THE MECHANISM OF HIF-1α STABILIZATION UNDER MODERATE HYPOXIA

The oxidative pentose phosphate cycle (OPPC) is necessary to maintain cellular reducing capacity during periods of increased oxidative stress. Metabolic flux through the OPPC increases stoichiometrically in response to a broad range of chemical oxidants, including those that generate reactive oxygen...

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Bibliographic Details
Published in:The Journal of biological chemistry 2007-12, Vol.282 (51), p.36790-36796
Main Authors: Tuttle, Stephen W, Maity, Amit, Oprysko, Patricia R, Kachur, Alexander V, Ayene, Iraimoudi S, Biaglow, John E, Koch, Cameron J
Format: Article
Language:English
Online Access:Get full text
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Summary:The oxidative pentose phosphate cycle (OPPC) is necessary to maintain cellular reducing capacity during periods of increased oxidative stress. Metabolic flux through the OPPC increases stoichiometrically in response to a broad range of chemical oxidants, including those that generate reactive oxygen species (ROS). Here we show that OPPC sensitivity is sufficient to detect low levels of ROS produced metabolically as a function of the percentage of O₂. We observe a significant decrease in OPPC activity in cells incubated under severe and moderate hypoxia (ranging from
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M700327200