Anatomy of ethylene-induced petal abscission in Pelargonium X hortorum

• Published in: Annals of botany 1993, Vol.71 (6), p.559-566
• Main Authors: Evensen, K.B, Page, A.M, Stead, A.D
• Format: Article
• Language: English
• Subjects: abscission; abscission zone; cell walls; corolla; ethylene; Pelargonium hortorum; plant anatomy; ultrastructure
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1006/anbo.1993.1072

When viewed under the light microscope, the abscission zone at the petal base of Pelargonium X hortorum consisted of small cells which, when stained with Toluidine Blue, possessed densely stained cells walls. After treatment with 1 microliter l-1 ethylene at 22 degrees C, the force required to separate the petals from the receptacle declined after a lag phase of only 30 min, with separation complete 60-90 min later depending upon the stage of development of the flower. Transmission electron micrographs of the petal abscission zones showed evidence of cell wall degradation, particularly in the middle lamella. These cells also contained extensive rough endoplasmic reticulum and numerous Golgi bodies and ribosomes. When abscission was complete, cells at the fracture face showed evidence of breakdown of cellular compartmentalization, often with little sign of an intact tonoplast. Scanning electron micrographs of recently-abscised surfaces showed that the epidermal cells surrounding the abscission zone were turgid and rounded, whereas those of the mesophyll cells were partially collapsed. The micrographic evidence is consistent with the hypothesis that ethylene-induced separation is caused by rapid enzymatic degradation of the cell walls.