Occurrence of Kabatina juniperi in Ontario and genetic analysis using RAPD markers

A survey conducted in 1992 and 1993 revealed that the major cause of juniper twig blight in Ontario was Kabatina juniperi. Phomopsis juniperovora was found in 3 of 26 samples received in 1992 and in none of 23 samples in 1993. Analysis of acervuli of K. juniperi collected from spring 1997 through sp...

Full description

Saved in:
Bibliographic Details
Published in:Canadian journal of plant pathology 2000-03, Vol.22 (1), p.79-88
Main Authors: Hsiang, T, Huang, J, Yang, L, Cook, S, Shen, Y, Richter, K.J
Format: Article
Language:English
Subjects:
blight
conidia
cultivars
disease transmission
epidemiology
fungal spores
genetic markers
genetic techniques and protocols
genetic variation
geographical variation
host range
incidence
Kabatina
linkage (genetics)
loci
molecular ecology
Phomopsis
population genetics
random amplified polymorphic DNA technique
seasonal variation
sporulation
strains
surveys
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A survey conducted in 1992 and 1993 revealed that the major cause of juniper twig blight in Ontario was Kabatina juniperi. Phomopsis juniperovora was found in 3 of 26 samples received in 1992 and in none of 23 samples in 1993. Analysis of acervuli of K. juniperi collected from spring 1997 through spring 1999 showed that spore production was high in spring, was lower in the summer, and gradually declined in the fall to very low levels in winter. Using RAPD markers, we found molecular variation between K. juniperi isolates from five locations within a 50 km radius. RAPD analysis of 24 isolates from five different cultivars of Juniperus scopulorum did not reveal host specialization. Among 49 isolates sampled from two adjacent rows of J. scopulorum 'Grey Gleam', there were 36 different RAPD haplotypes. Genetic diversity analysis of this population showed significant gametic linkage disequilibrium between putative RAPD loci and indicated that asexual propagules such as conidia are the major form of dispersal of this pathogen. A practical implication of this work is that pruning operations should be timed so that they do not coincide with peak spore production.
ISSN:0706-0661
1715-2992
DOI:10.1080/07060660009501164