Exogenous Cytokinin Treatments of an NI Hyper-Accumulator, Alyssum Murale, Grown in a Serpentine Soil: Implications for Phytoextraction

• Published in: International journal of phytoremediation 2011, Vol.13 (1), p.90-101
• Main Authors: Cassina, L, Tassi, E, Morelli, E, Giorgetti, L, Remorini, D, Chaney, R. L, Barbafieri, M
• Format: Article
• Language: English
• Subjects: Accumulation; Alyssum murale; Biodegradation, Environmental; Biomass; Brassicaceae - drug effects; Brassicaceae - growth & development; Brassicaceae - metabolism; Cytokinins; Cytokinins - pharmacology; exogenous cytokinins; Gas exchange; Glutathione; Growth regulators; hyperaccumulators; metal hyperaccumulator; Metals, Heavy - analysis; nickel; Nickel - analysis; Nickel - metabolism; phytochelatin; Phytochelatins; phytoremediation; Plant biomass; Plant extracts; Plant growth; plant growth regulators; Plant Growth Regulators - pharmacology; Plant Leaves - metabolism; Plant species; Plant Stomata - metabolism; Plant Transpiration; polluted soils; Protein thiols; Secologanin Tryptamine Alkaloids; Serpentine; serpentine soil; Serpentine soils; Soil - chemistry; Soil contamination; Soil Pollutants - metabolism; Soil pollution; Soils; Stomata; stomatal movement; Thiols; Transpiration; transpiration rate; Water - metabolism
• ISSN: 1549-7879; 1522-6514; 1549-7879
• DOI: 10.1080/15226514.2011.568538

Application of exogenous plant growth regulators was examined as a viable technique to increase the efficiency of plant metal extraction from contaminated soils. The aim of this study was to investigate the alteration of Ni phytoextraction by Alyssum murale, a Ni hyperaccumulator, following the application of cytokinins. The following parameters were investigated: Ni accumulation, plant growth, gas exchange, stomata behavior and the concentration of nonprotein thiols (glutathione, y-Glu-Cys, and phytochelatins). In a pot experiment, A. murale plants grown in a serpentine soil were treated with a mix of naturally occurring cytokinins. Results showed that Ni accumulation in plants ranged from 4000 to 7000 mg kg⁻¹ confirming the hyper-accumulation ability from the soil used. Cytokinin treatments produced a significant increase in plant biomass and transpiration rate whereas no significant variation in Ni accumulation or the concentration of non-protein thiols was observed. The results suggest that A. murale is a plant species sensitive to cytokinin treatment and that cytokinin treatment is potentially useful in increasing the phytoextraction capability by increasing biomass. Moreover, for first time, evidence was obtained that the Ni hyperaccumulation mechanism is independent of water flux and transpiration rate.