As(V)-reduction to As(III) by arsenic-resistant Bacillus spp. bacterial strains isolated from low-contaminated sediments of the Oliveri-Tindari Lagoon, Italy

• Published in: Chemistry and ecology 2011-06, Vol.27 (3), p.207-219
• Main Authors: Ruta, Marcella, Pepi, Milva, Gaggi, Carlo, Bernardini, Emanuele, Focardi, Silvia, Magaldi, Eleonora, Gasperini, Simone, Volterrani, Margherita, Zanini, Angiola, Focardi, Silvano E
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Applied ecology; arsenic; Bacillus; Bacillus (bacteria); Biological and medical sciences; Brackish; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; General aspects; growth retardation; lakes; minimum inhibitory concentration; nucleotide sequences; phylogeny; resistance to arsenic; ribosomal RNA; sediments
• ISSN: 1029-0370; 0275-7540; 1029-0370
• DOI: 10.1080/02757540.2010.547490

Five arsenic-resistant bacterial strains designated MT1, MT2, MT3, V1 and V2 were isolated from sediments of the Oliveri-Tindari Lagoon (Italy), which comprises six small lakes whose sediments contain low arsenic concentrations. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genus Bacillus. Bacillus sp. strain MT3 showed higher tolerance to As(III) and As(V), as indicated by minimum inhibitory concentrations of 14 and 135 mmol⁻¹, respectively. Bacillus sp. strain V1 showed growth inhibition at 14 mmol⁻¹ in the presence of As(III) and at 68 mmol⁻¹ in the presence of As(V), whereas the arsenic resistance of Bacillus sp. strain MT1 was 10 and 27 mmol⁻¹ for As(III) and As(V), respectively. The strains Bacillus spp. MT2 and V2 showed low levels of As(III) and As(V) resistance, as it was unable to grow at concentrations>7 and 14 mmol⁻¹, respectively. The isolated arsenic-resistant Bacillus spp. strains were able to reduce As(V) to As(III), especially Bacillus spp. strain MT3. This study suggests that the isolated bacterial strains play a role in the arsenic biogeochemical cycle of arsenic-poor sediments in the Oliveri-Tindari Lagoon.