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Colonization and virulence factors of methicillin resistant Staphylococcus aureus in a pediatric population in Monteria, Colombia/Colonización y factores de virulencia de Staphylococcus aureus resistente a meticilina en una población infantil de Montería/Colonizacao e fatores de virulencia de Staphylococcus aureus resistente a meticilina numa populacao infantil de Monteria
Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is able to colonize the human body, most frequently the nostrils, but also the hands, perineum and throat. Such colonization has been proposed as a risk factor to acquire future infections. Objective: To determine the prevalence, and t...
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Published in: | Iatreia (Medellín, Colombia) Colombia), 2015-07, Vol.28 (3), p.259 |
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Main Authors: | , , , |
Format: | Article |
Language: | Spanish |
Online Access: | Get full text |
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Summary: | Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is able to colonize the human body, most frequently the nostrils, but also the hands, perineum and throat. Such colonization has been proposed as a risk factor to acquire future infections. Objective: To determine the prevalence, and the microbiological and molecular characteristics of MRSA in healthy children. Methodology: A cross-sectional descriptive study was done of 150 children from 13 day care centers in Monteria. Colombia. Nasal and throat swabs were obtained. The isolates were identified and characterized by microbiological and molecular methods. Results: The MRSA colonization rate was 9.3% (14/150). 62.5% of the isolates carried the subtype IVc of SCCmec, and 87.5% had the genes encoding for PVL and Sefe, while 81.2% carried the gene bsaB. Conclusion: The percentage of colonization found is one of the highest reported among children from the Colombian Caribbean region, and the isolates have virulence factors that have been associated with an aggressive clinical course. KEY WORDS Child; Child Day Care Centers; Methicillin resistant Staphylococcus aureus; Virulence Factors Introduccion: Staphylococcus aureus resistente a meticilina (SARM) puede colonizar el cuerpo humano, con mayor frecuencia en las fosas nasales, pero tambien en las manos, el perine y la faringe. Ademas, se ha propuesto que la colonizacion puede ser un factor de riesgo para adquirir infecciones futuras. Objetivo: determinar la prevalencia y las caracteristicas microbiologicas y moleculares del SARM en una poblacion infantil sana. Metodologia: se hizo un estudio descriptivo transversal para determinar la tasa de colonizacion nasal por SARM en 150 ninos pertenecientes a 13 hogares infantiles de la ciudad de Monteria. Los aislamientos se hicieron a partir de hisopados nasales y faringeos, se identificaron mediante pruebas microbiologicas convencionales y se confirmaron y caracterizaron por metodos moleculares. Resultados: la tasa de colonizacion por SARM fue del 9,3% (14/150). El 62,5% de los aislamientos portaban el SCCmee subtipo IVc; 87,5% de los aislamientos presentaron los genes que codifican para PVL y Sek. mientras que 81,2% portaban el gen bsaB. Conclusion: el porcentaje de colonizacion hallado es uno de los mas altos reportados para la poblacion infantil de la region Caribe colombiana, y ios aislamientos presentaron factores de virulencia relacionados con cuadros clinicos agresivos. PALABRAS CLAVE Factores de V |
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ISSN: | 0121-0793 |