Poly

Poly(ADP-ribosyl)ation regulates chromatin structure and transcription driving epigenetic events. In particular, Parp1 is able to directly influence DNA methylation patterns controlling transcription and activity of Dnmt1. Here, we show that ADP-ribose polymer levels and Parp1 expression are noticea...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e46927
Main Authors: Calabrese, Roberta, Catizone, Angela, Zampieri, Michele, Ciccarone, Fabio, Bacalini, Maria Giulia, De Felici, Massimo, Caiafa, Paola, Klinger, Francesca Gioia
Format: Article
Language:English
Subjects:
Chromatin
DNA
DNA damage
DNA repair
Epigenetic inheritance
Genes
Genomes
Genomics
Methylation
Methyltransferases
Monosaccharides
Transcription (Genetics)
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Poly(ADP-ribosyl)ation regulates chromatin structure and transcription driving epigenetic events. In particular, Parp1 is able to directly influence DNA methylation patterns controlling transcription and activity of Dnmt1. Here, we show that ADP-ribose polymer levels and Parp1 expression are noticeably high in mouse primordial germ cells (PGCs) when the bulk of DNA demethylation occurs during germline epigenetic reprogramming in the embryo. Notably, Parp1 activity is stimulated in PGCs even before its participation in the DNA damage response associated with active DNA demethylation. We demonstrate that PARP inhibition impairs both genome-wide and locus-specific DNA methylation erasure in PGCs. Moreover, we evidence that impairment of PARP activity causes a significant reduction of expression of the gene coding for Tet1 hydroxylases involved in active DNA demethylation. Taken together these results demonstrate new and adjuvant roles of poly(ADP-ribosyl)ation during germline DNA demethylation and suggest its possible more general involvement in genome reprogramming.
ISSN:1932-6203
1932-6203