Poly

Potentiodynamically fabricated poly(alizarin red s) modified GCE was characterized using CV and EIS techniques. In contrast to the cyclic voltammetric response of the unmodified GCE for metronidazole, an irreversible reduction peak with three-folds of current enhancement and reduced overpotential at...

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Bibliographic Details
Published in:PloS one 2020-12, Vol.15 (12), p.e0244115
Main Authors: Turbale, Mahilet, Sailor, Girish, Amare, Meareg, Dawit, Mulugeta, Moges, Amsalu
Format: Article
Language:English
Subjects:
Drug metabolism
Dyes and dyeing
Electrodes
Metronidazole
Pharmacology, Experimental
Testing
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Summary:Potentiodynamically fabricated poly(alizarin red s) modified GCE was characterized using CV and EIS techniques. In contrast to the cyclic voltammetric response of the unmodified GCE for metronidazole, an irreversible reduction peak with three-folds of current enhancement and reduced overpotential at the poly(alizarin red s) modified GCE showed the catalytic effect of the modifier towards reduction of metronidazole. While observed peak potential shift with increasing pH (4.0-10.0) indicated the involvement of protons during the reduction of metronidazole, peak potential shift with scan rate (20-300 mV s.sup.-1) confirmed the irreversibility of the reduction reaction of metronidazole at the modified GCE. A better correlation for the dependence of peak current on scan rate (r.sup.2 = 0.9883) than on square root of scan rate (r.sup.2 = 0.9740) supplemented by slope value of 0.38 for plot of log(current) versus log(scan rate) indicated the reduction reaction of metronidazole at the surface of the modified electrode was predominantly adsorption controlled. Under the optimized method and solution parameters, reductive current response of tablet sample showed linear dependence on spiked standard concentration in a wide range (0-125 [mu]M) with excellent determination coefficient r.sup.2, LoD and LoQ of 0.9991, 0.38, and 1.25 [mu]M, respectively. Spike recovery of 97.9% and interference recovery of 96.2-97.5% in the presence of 21.28 and 31.92 [mu]M of uric acid and ascorbic acid validated the applicability of the present method for determination of metronidazole in tablet formulation. The metronidazole content of the tested tablet formulation using standard addition method was found to be 97.6% of what is claimed by the tablet manufacturer making the developed method an excellent potential candidate for its applicability to determine metronidazole in real samples with complex matrix.
ISSN:1932-6203
1932-6203