Use PCR & a Single Hair to Produce a "DNA Fingerprint"

Presents a laboratory procedure that involves students extracting their own DNA from a single hair follicle, using the polymerase chain reaction (PCR) to amplify a polymorphic locus, performing electrophoresis on the PCR products on an agarose gel, and visualizing the alleles to generate a "DNA...

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Published in:The American biology teacher 1997-03, Vol.59 (3), p.172-178
Main Authors: Campbell, A. Malcolm, John H. Williamson, Diane Padula, Steve Sundby
Format: Article
Language:English
Subjects:
Alleles
Biology
Biotechnology
Bromides
DNA
DNA identification
DNA testing
Follicles
Gels
Genetic loci
Genetics
Hair
Hair follicles
Higher Education
How-To-Do-It
Laboratory Equipment
Polymerase chain reaction
Science Experiments
Science Instruction
Study and teaching
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cited_by cdi_FETCH-LOGICAL-c310t-1397b3d5d0aaf0ac83b73cb18f50509b8b7c8ff634f29ad07a8674153b7ecc2f3
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container_end_page 178
container_issue 3
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container_title The American biology teacher
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creator Campbell, A. Malcolm
John H. Williamson
Diane Padula
Steve Sundby
description Presents a laboratory procedure that involves students extracting their own DNA from a single hair follicle, using the polymerase chain reaction (PCR) to amplify a polymorphic locus, performing electrophoresis on the PCR products on an agarose gel, and visualizing the alleles to generate a "DNA fingerprint." Discusses theoretical background, teaching applications, timetable and equipment, beginner's protocol, and advanced protocol. (JRH)
doi_str_mv 10.2307/4450275
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ispartof The American biology teacher, 1997-03, Vol.59 (3), p.172-178
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source JSTOR Archival Journals and Primary Sources Collection; ERIC
subjects Alleles
Biology
Biotechnology
Bromides
DNA
DNA identification
DNA testing
Follicles
Gels
Genetic loci
Genetics
Hair
Hair follicles
Higher Education
How-To-Do-It
Laboratory Equipment
Polymerase chain reaction
Science Experiments
Science Instruction
Study and teaching
title Use PCR & a Single Hair to Produce a "DNA Fingerprint"
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