Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp

• Published in: PloS one 2020-04, Vol.15 (4), p.e0220485
• Main Authors: Delahaut, Vyshal, Raskovic, Bozidar, Salvado, Marta Satorres, Bervoets, Lieven, Blust, Ronny, De Boeck, Gudrun
• Format: Article
• Language: English
• Subjects: Analysis; Cadmium; Edema; Electrolytes; Epithelium; Fishes; Hypertrophy; Toxicity
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0220485

The individual toxicity and bioaccumulation of cadmium, copper and zinc for common carp juveniles was evaluated in a direct comparison in two experimental setups. First, fish were exposed for 10 days to different metal concentrations in order to link metal bioaccumulation to LC.sub.50 values (concentration lethal to 50% of the animals) and incipient lethal levels (ILL, concentration where 50% survives indefinitely). Accumulated metals showed a positive dose dependent uptake for cadmium and copper, but not for zinc. Toxicity was in the order cadmium>copper>zinc with 96h LC.sub.50 values for cadmium at 0.20±0.16 [mu]M, for copper at 0.77±0.03 [mu]M, and for zinc at 29.89±9.03 [mu]M respectively. For copper, the 96h exposure was sufficient to calculate the incipient lethal level and therefore 96h LC.sub.50 and ILL levels were the same, while for cadmium and zinc 5 to 6 days were needed to reach ILL resulting in slightly lower values at 0.16 [mu]M and 28.33 [mu]M respectively. Subsequently, a subacute exposure experiment was conducted, where carp juveniles were exposed to 2 equitoxic concentrations (10% and 50% of LC.sub.50 96 h) of the three metals for 1, 3 and 7 days. Again a significant dose-dependent increase in gill cadmium and copper, but not in zinc, was observed during the 7-day exposure. Copper clearly affected sodium levels in gill tissue, while zinc and cadmium did not significantly alter any of the gill electrolytes. The overall histopathological effects (e.g. hyperemia and hypertrophy) of the metal exposures were mild for most of the alterations. Our study showed that copper an cadmium (but not zinc) showed dose dependent metal accumulation, however this bioaccumulation was only correlated with mortality for cadmium. Metal specific alterations were reduced gill sodium levels in copper exposed fish and oedema of the primary epithelium which typically occurred in both levels of zinc exposure.