Biochemical and molecular characterization of Lactobacillus spp. isolated from the ileum of broilers treated with or without antimicrobials/Identificacao bioquimica e molecular de Lactobacillus spp. isolados do ileo de frangos de corte tratados ou nao com antimicrobianos

• Published in: Ciência rural 2012-09, Vol.42 (9), p.1648
• Main Authors: Zanini, Surama Freitas, Mussi, Jamili Maria Suhet, Zanini, Marcos Santos, Sousa, Dyeime Ribeiro, Pessotti, Bruna Mirelly de Souza, Damasceno, Joao Damasceno Lopes Ma, da Silva, Maria Aparecida
• Format: Article
• Language: Spanish
• ISSN: 0103-8478

This study aimed to characterize biochemically and molecular species of Lactobacillus spp. isolated from the ileum of broiler chickens treated with or without antimicrobial. A total of 400 day-old male chicks, Cobb, distributed in a randomized design in groups of five treatments and five replicates: diet without antimicrobials; diet with antimicrobials; diet with 0.4% Brazilian red pepper oil (BRP); diet with 200mg vitamin E [kg.sup.-1]; diet with 0.4% BRP and 200mg vitamin E [kg.sup.-1]. The biochemical results have identified L. acidophilus, L. fermentum, L. plantarum, L. delbrueckii subsp. delbrueckii besides Lactococcus lactis subp. Lactis. For samples ATCC, the biochemical identification raised some doubts about their results. The molecular identification showed that Lactobacillus that amplified both primers (LU-1 '/ Lac-2) and (Laci-1 / 23-10(C) are the species L. acidophilus. However, samples that amplified only with primer (LU-1 '/ Lac-2) are other species that constitute the group L. acidophilus. However, the samples with the primer L. fermentum (Fer 3/Fer 4) amplified a 192pb fragment pattern for this species. We conclude that the identification of species of Lactobacillus isolated from the ileum of PCR was more sensitive than the biochemical method. Key words: broilers, antibiotics, Schinus terebinthifolius Raddi, essential oil, PCR. Objetivou-se caracterizar bioquimicamente e molecularmente as especies de Lactobacillus spp. isoladas do ileo de frangos de corte tratados ou nao com antimicrobianos. Utilizou-se 400 pintos de corte alojados em 25 boxes de 2[m.sup.2] (16 aves/boxe), distribuidos em um delineamento inteiramente casualizado, em grupos de cinco tratamentos e cinco repeticoes: dieta sem promotor de crescimento; dieta com promotor de crescimento; dieta com 0,4% de oleo de aroeira-vermelha (OAV); dieta com 200mg de vitamina E [kg.sup.-1]; dieta com 0,4% OAV e 200mg de vitamina E [kg.sup.-1]. Apos a caracterizacao fenotipica do genero Lactobacillus, foram identificadas, em ambas as metodologias, 100 amostras de Lactobacillus spp. sendo 20 amostras por tratamento. Os resultados bioquimicos identificaram L. acidophilus, L. fermentum, L. plantarum, L. delbrueckii subsp. delbrueckii alem de Lactococcus Lactis subp. lactis. Para as amostras padrao ATCC, a identificacao bioquimica suscitou algumas duvidas em relacao aos seus resultados. Os resultados da identificacao molecular mostraram que os Lactobacillus que amplificaram ambos os iniciadores