Antimicrobial activity of spice essential oils against Escherichia coli strains isolated from poultry and cattle/Atividade antimicrobiana de oleos essenciais de condimentos frente a amostras de Escherichia coli isoladas de aves e bovinos

• Published in: Ciência rural 2011-06, Vol.41 (6), p.1051
• Main Authors: Santurio, Deise Flores, da Costa, Mateus Matiuzzi, Maboni, Grazieli, Cavalheiro, Carlos Pasqualin, de Sa, Mariangela Facco, Dal Pozzo, Marcelo, Alves, Sydney Hartz, Fries, Leadir Lucy Ma
• Format: Article
• Language: Spanish
• Subjects: Antibiotics; Escherichia coli; Feed industry; Poultry industry
• ISSN: 0103-8478

The discussed issue about replacing the use of antibiotics in animal feed (growth promoters) requires emerging alternatives. To meet the needs of microbial inhibitors in these foods, the essentials oils (EOS) constitute potential alternatives under evaluation. In this study it was evaluated the antimicrobial activities of EOs from Oreganum vulgare (oregano), Thymus vulgaris (thyme), Lippia graveolens (Mexican oregano), Cinnamomum zeylanicum (cinnamon), Zingiber officinale (ginger), Salvia officinalis (sage), Rosmarinus officinalis (rosemary) and Ocimum basilicum (basil) against Escherichia coli strains isolated from poultry (n=43) and cattle faeces (n=36). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for each isolate by using the broth microdilution technique, from the maximum concentration of 6400[micro]g [mL.sup.-1] of each OE tested. Antimicrobial activity was observed on the essential oils of oregano, mexican oregano, thymus and cinnamon. For all strains tested, regardless of their origin, the OES more and less effective as antimicrobial activity were oregano and cinnamon, respectively. These results confirm the antimicrobial potential of some EOs, which deserve further research, addressing the addition of essential oils in poultry and cattle feeding. Key words: Escherichia coli, essential oils, antimicrobial activity. A discutida questao da substituicao do uso de antibacterianos em racoes (promotores de crescimento) requer urgentes alternativas. Face as necessidades de inibidores microbianos nesses alimentos, os oleos essenciais (OES) se constituem em alternativa, sob avaliacao. Neste estudo, avaliouse a atividade antimicrobiana dos OES de Origanum vulgare (oregano), Thymus vulgaris (tomilho), Cinnamomum zeylanicum (canela), Lippia graveolens (oregano mexicano), Zingiber officinale (gengibre), Salvia officinalis (salvia), Rosmarinus officinalis (alecrim) e Ocimum basilicum (manjericao) frente a amostras de Escherichia coli isoladas de fezes de aves (n=43) e de bovinos (n=36). A concentracao inibitoria minima (CIM) e a concentracao bactericida minima (CBM) foram determinadas para cada isolado atraves da tecnica de microdiluicao em caldo, a partir da maxima concentracao de 6400[micro]g [mL.sup.-1] de cada OE testado. Observouse atividade antimicrobiana para os OES de oregano, oregano mexicano, tomilho, canela. Para todas as amostras testadas, independente de sua origem, os OES mais e menos efeti