Maintenance of human peripheral blood mononuclear cell viability in propolis extracts and formulations/Manutencao da viabilidade das celulas mononucleares de sangue periferico humano em extratos e formulacoes de propolis

• Published in: Acta scientiarum. Health sciences 2012-01, Vol.34 (1), p.60
• Main Authors: Casaroto, Ana Regina, Sell, Ana Maria, Nagata, Juliana Yuri, Brunetta, Elaine Vasconcelos, Franco, Selma Lucy, Hidalgo, Mirian Marubayashi
• Format: Article
• Language: Spanish
• ISSN: 1679-9291
• DOI: 10.4025/actascihealthsci.v34i1.8954

In this study a comparison was made of human mononuclear cell (PBMCs) viability, when cells were kept in different propolis extracts and formulations. PBMCs ([10.sup.6] cell [mL.sup.-1]), obtained from healthy donors (n=5), were incubated at 20°C in the different propolis solutions, as well as in Hank's balanced salt solution (HBSS), used as experimental control. The cell viability was analyzed by Trypan blue exclusion assay. When incubated for 1h, only two extracts, denominated A70D and D70D, showed appropriate results for maintaining cell viability. A70D and D70D showed better viability (p < 0.05) than other formulations and no difference (p > 0.05) from the HBSS control. A70D and D70D were tested in five dilutions in propylene glycol, over 24h, with analysis at 0, 30 minutes, 1, 3, 6, 10 and 24h. The most concentrated fractions showed the worst performance (p < 0.05) in comparison with their original extracts, which remained close to 80% viability over 24h. Reduction in viability proportional to increase in concentration of formulations was observed. The results suggest that propolis solutions in appropriate concentrations may be used in future studies on alternatives to mediums routinely used in dental practice for storing avulsed teeth. Keywords: propolis, cell viability, tooth avulsion, tooth replantation. Neste estudo comparou-se a viabilidade das celulas mononucleares de sangue periferico (PBMCs) humano quando mantidas em diferentes extratos e formulacoes de propolis. As PBMCs ([10.sup.6] cels [mL.sup.-1]), provenientes de doadores saudaveis (n=5), foram estocadas a 20°C nas diferentes solucoes de propolis, assim como em solucao salina balanceada de Hank's (HBSS), utilizada como controle do experimento. A viabilidade celular foi determinada pelo metodo de exclusao com azul de Tripan. Quando incubadas por 1h, apenas dois extratos, denominados A70D e D70D, apresentaram desempenho satisfatorio na manutencao da viabilidade celular, semelhante (p > 0,05) ao controle HBSS e diferindo estatisticamente (p < 0,05) das demais formulacoes. A70D e D70D foram entao testados em cinco diluicoes em propilenoglicol, ao longo de 24h, com analise nos tempos 0, 30 min., 1, 3, 6, 10 e 24h. As fracoes mais concentradas apresentaram pior desempenho (p < 0,05) em relacao aos seus extratos originais que mantiveram viabilidade proxima a 80% ao longo de 24h. A reducao da viabilidade proporcional ao aumento da concentracao das fracoes foi observada. Os resultados sugerem que