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Agro-industrial residues utilization of palm oil for lipase production by Aspergillus niger/Aproveitamento de residuos da agroindustria do oleo de dende para a producao de lipase por Aspergillus niger
In this paper it was evaluated the performance of two strains of Aspergillus niger (mutant 11T53A14 and wild type C) previously selected as promising for lipase production, from cultivation media formulated from palm kernel cake (kernel) and alkaline sludge from refining were evaluated palm oil (pal...
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Published in: | Ciência rural 2016-04, p.755 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | Spanish |
Online Access: | Get full text |
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Summary: | In this paper it was evaluated the performance of two strains of Aspergillus niger (mutant 11T53A14 and wild type C) previously selected as promising for lipase production, from cultivation media formulated from palm kernel cake (kernel) and alkaline sludge from refining were evaluated palm oil (palm oil sludge), palm oil (palm) waste industrialization by solid state fermentation (SSF). Experiments of enzyme production in SSF were conducted in aerated columns, incubated in a water bath at 32°C with controlled inlet of 1.0vvm. The best results were obtained with the mutant strain A. niger 11T53A14 in medium containing palm kernel cake moistened with a solution of ammonium sulfate (1.2%) and with the addition of 3% of palm oil sludge. The maximum lipase activity in this medium was 72.57U [g.sub.dw.sup.-1] in 48 hours and 47.5% higher than in the medium without sludge palm. A comparison of the performance of the mutant strain with the wildtype strain showed that the medium composed of palm kernel cake added with ammonium sulfate and blurs palm induced both strains to produce lipases with good activity levels and reduced the time of the fermentation process. Key words: lipase, agro-industrial residue, Aspergillus niger. Neste trabalho, foi avaliado o desempenho de duas linhagens de Aspergillus niger (mutante HT53A14 e selvagem C) previamente selecionadas como promissoras para a producao de lipases, utilizando meios de cultivo formulados a partir da torta de dende (palmiste) e da borra alcalina do refino do oleo de dende (borra de dende), residuos provenientes da agroindustria do oleo de dende (palma) por fermentacao em estado solido (FES). Os experimentos de producao da enzima em FES foram conduzidos em colunas aeradas, incubadas em banho-maria a 32°C com entrada controlada de ar nao umedecido de l,0vvm. Os melhores resultados foram obtidos com a cepa mutante A. niger 11T53A14 em meio contendo torta de dende umedecida com solucao sulfato de amonio (1,2%) e com a adicao de 3% da borra de dende. O valor maximo da atividade da lipase neste meio foi de 72,57U [g.sup.dw.sup.-1] em 48 horas. Esse valor foi 47,5% superior ao obtido no meio sem a borra de dende. A comparacao do desempenho da cepa mutante com a cepa selvagem mostrou que o meio composto por torta de dende adicionado com sulfato de amonio e borra de dende induziu ambas as linhagens a produzir lipases com bons niveis de atividade, alem de reduzir o tempo de processo de fermentacao. Palavras-chave: lipase, |
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ISSN: | 0103-8478 |
DOI: | 10.1590/0103-8478cr20131673 |