A phosphatase complex that dephosphorylates γH2AX regulates DNA damage checkpoint recovery

One of the earliest marks of a double-strand break (DSB) in eukaryotes is serine phosphorylation of the histone variant H2AX at the carboxy-terminal SQE motif to create γH2AX-containing nucleosomes. Budding-yeast histone H2A is phosphorylated in a similar manner by the checkpoint kinases Tel1 and Me...

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Published in:Nature 2006-01, Vol.439 (7075), p.497-501
Main Authors: Lieberman, Judy, Keogh, Michael-Christopher, Downey, Michael, Chowdhury, Dipanjan, Datta, Nira, Harrison, Jacob C, Emili, Andrew, Haber, James E, Krogan, Nevan J, Durocher, Daniel, Fillingham, Jeffrey, Greenblatt, Jack F, Galicia, Sarah, Shen, Xuetong, Buratowski, Stephen, Kim, Jung-Ae, Onishi, Megumi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Humanities and Social Sciences
letter
Molecular and cellular biology
Molecular genetics
multidisciplinary
Mutagenesis. Repair
Science
Science (multidisciplinary)
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Summary:One of the earliest marks of a double-strand break (DSB) in eukaryotes is serine phosphorylation of the histone variant H2AX at the carboxy-terminal SQE motif to create γH2AX-containing nucleosomes. Budding-yeast histone H2A is phosphorylated in a similar manner by the checkpoint kinases Tel1 and Mec1 (ref. 2; orthologous to mammalian ATM and ATR, respectively) over a 50-kilobase region surrounding the DSB. This modification is important for recruiting numerous DSB-recognition and repair factors to the break site, including DNA damage checkpoint proteins, chromatin remodellers and cohesins. Multiple mechanisms for eliminating γH2AX as DNA repair completes are possible, including removal by histone exchange followed potentially by degradation, or, alternatively, dephosphorylation. Here we describe a three-protein complex (HTP-C, for histone H2A phosphatase complex) containing the phosphatase Pph3 that regulates the phosphorylation status of γH2AX in vivo and efficiently dephosphorylates γH2AX in vitro. γH2AX is lost from chromatin surrounding a DSB independently of the HTP-C, indicating that the phosphatase targets γH2AX after its displacement from DNA. The dephosphorylation of γH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature04384