Mass spectrometry in the study of extracellular enzymes produced by filamentous fungi

The use of MALDI-TOF and other types of mass spectrometry for the identification and investigation of extracellular enzymes (carbohydrases, proteinases, esterases, etc.) produced by filamentous fungi belonging to the genera Aspergillus, Trichoderma, Penicilium, Chrysosporium , etc. is discussed. The...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2010-12, Vol.65 (14), p.1446-1461
Main Authors: Gusakov, A. V., Semenova, M. V., Sinitsyn, A. P.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Fungi
Internet software
Peptides
Proteases
Reviews
Spectrum analysis
Toy industry
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Summary:The use of MALDI-TOF and other types of mass spectrometry for the identification and investigation of extracellular enzymes (carbohydrases, proteinases, esterases, etc.) produced by filamentous fungi belonging to the genera Aspergillus, Trichoderma, Penicilium, Chrysosporium , etc. is discussed. The method of mass spectrometric peptide fingerprinting combined with the use of Internet software for on line data analysis (MASCOT, Aldente, FindPept, FindMod, GlycoMod) allows the fast and reliable identification of both individual enzymes and the components of crude multienzyme preparations without their fractionation. The method was also applied to the discrimination of the fungal genes encoding enzymes with similar substrate specificity. Other enzyme-based applications of mass spectrometry, such as the revelation of the presence or absence of structural domains in the molecules of carbohydrases, the detection of posttranslational and artificial modifications in the enzymes, and the use of tandem mass spectrometry for de novo peptide sequencing are described.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934810140030