Nitration of [beta]-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice

We revealed in previous studies that nitration of food proteins reduces the risk of de novo sensitization in a murine food allergy model. In contrast, in situations with preformed specific IgE antibodies, in vitro experiments suggested an increased capacity of effector cell activation by nitrated fo...

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Bibliographic Details
Published in:PloS one 2015-05, Vol.10 (5)
Main Authors: Diesner, Susanne C, Schultz, Cornelia, Ackaert, Chloé, Oostingh, Gertie J, Ondracek, Anna, Stremnitzer, Caroline, Singer, Josef, Heiden, Denise, Roth-Walter, Franziska, Fazekas, Judit, Assmann, Vera E, Jensen-Jarolim, Erika, Stutz, Hanno, Duschl, Albert, Untersmayr, Eva
Format: Article
Language:English
Subjects:
Analysis
Causes of
Development and progression
Food hypersensitivity
Milk proteins
Nitration
Physiological aspects
Properties
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Summary:We revealed in previous studies that nitration of food proteins reduces the risk of de novo sensitization in a murine food allergy model. In contrast, in situations with preformed specific IgE antibodies, in vitro experiments suggested an increased capacity of effector cell activation by nitrated food proteins. The aim of this study was to investigate the influence of protein nitration on the effector phase of food allergy. BALB/c mice were immunized intraperitoneally (i.p.) with the milk allergen [beta]-lactoglobulin (BLG) or the egg allergen ovomucoid (OVM), followed by intragastric (i.g.) gavages to induce a strong local inflammatory response and allergen-specific antibodies. Subsequently, naïve and allergic mice were intravenously (i.v.) challenged with untreated, sham-nitrated or nitrated BLG or OVM. Anaphylaxis was monitored by measuring core body temperature and determination of mouse mast cell protease-1 (mMCP-1) levels in blood. A significant drop of body temperature accompanied with significantly elevated concentrations of the anaphylaxis marker mMCP-1 were only observed in BLG allergic animals challenged with nitrated BLG and not in OVM allergic mice challenged with nitrated OVM. SDS-PAGE and circular dichroism analysis of the differentially modified allergens revealed an effect of nitration on the secondary protein structure exclusively for BLG together with enhanced protein aggregation. Our data suggest that nitration affects differently the food allergens BLG and OVM. In the case of BLG, structural changes favored dimerization possibly explaining the increased anaphylactic reactivity in BLG allergic animals.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0126279