No Differential Regulation of Dopamine Transporter Binding in a Primate Model of Parkinson Disease

Radioligands for DAT and VMAT2 are widely used presynaptic markers for assessing dopamine (DA) nerve terminals in Parkinson disease (PD). Previous in vivo imaging and postmortem studies suggest that these transporter sites may be regulated as the numbers of nigrostriatal neurons change in pathologic...

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Bibliographic Details
Published in:PloS one 2012-02, Vol.7 (2), p.e31439
Main Authors: Tian, LinLin, Karimi, Morvarid, Loftin, Susan K, Brown, Chris A, Xia, HuChuan, Xu, JinBin, Mach, Robert H, Perlmutter, Joel S
Format: Article
Language:English
Subjects:
Dopamine
Medical research
Neurons
Phenols (Class of compounds)
Primates
Tyrosine
Online Access:Get full text
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Summary:Radioligands for DAT and VMAT2 are widely used presynaptic markers for assessing dopamine (DA) nerve terminals in Parkinson disease (PD). Previous in vivo imaging and postmortem studies suggest that these transporter sites may be regulated as the numbers of nigrostriatal neurons change in pathologic conditions. To investigate this issue, we used in vitro quantitative autoradioradiography to measure striatal DAT and VMAT2 specific binding in postmortem brain from 14 monkeys after unilateral internal carotid artery infusion of 1-Methyl-4-Phenyl-1,2,3,6-tetrahydropyridine (MPTP) with doses varying from 0 to 0.31 mg/kg. Quantitative estimates of the number of tyrosine hydroxylase (TH)-immunoreactive (ir) neurons in substantia nigra (SN) were determined with unbiased stereology, and quantitative autoradiography was used to measure DAT and VMAT2 striatal specific binding. Striatal VMAT2 and DAT binding correlated with striatal DA (r.sub.s = 0.83, r.sub.s = 0.80, respectively, both with n = 14, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0031439