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Quantification and analysis of the viability of cistos de protozoarios patogenicos em lodo de esgoto

For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer th...

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Bibliographic Details
Published in:Acta scientiarum. Technology 2018-01, Vol.40 (1)
Main Authors: Medeiros, Raphael Correa, Daniel, Luiz Antonio
Format: Article
Language:Spanish
Online Access:Get full text
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Summary:For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts. Keywords: Cryptosporidium, Giardia, activated sludge, reuse. Com vista a utilizacao de subprodutos do tratamento de esgoto sanitario, a questao microbiologica do lodo de esgoto e extremamente importante, pois revela riscos diretos e indiretos a saude publica quanto a sua manipulacao e uso inadequados. Este trabalho buscou quantificar cistos de Giardia spp. e oocistos de Cryptosporidium spp., bem como inferir a sua viabilidade, em amostras de lodo de duas estacoes de tratamento de esgoto. Foi utilizado metodo com tres centrifugacoes sucessivas, seguido de reacao de imunofluorescencia direta e coloracao com iodeto de propideo. As amostras foram 100% (14/14) positivas para cistos e 57,1% (8/14) para oocistos, com elevadas concentracoes e viabilidade media acima de 75%. A mudanca do sistema de tratamento de lodos ativados, de aeracao prolongada para convencional, nao mostrou diferenca na concentracao e na viabilidade dos (oo)cistos. Palavras-chave: Cryptosporidium, Giardia, lodos ativados, reuso.
ISSN:1806-2563
DOI:10.4025/actascitechnol.v40i1.28709