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DNA AGEs and aging

INTRODUCTION: Aging is a syndrome of deleterious, progressive, universal and thus far irreversible changes both at molecular level, cell and organ level. Glycation is the addition of reducing sugars and related compounds to proteins, DNA and lipids. Glycation theory of aging suggests that modificati...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.33 (S1), p.S62
Main Author: Kahkasha
Format: Article
Language:English
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Summary:INTRODUCTION: Aging is a syndrome of deleterious, progressive, universal and thus far irreversible changes both at molecular level, cell and organ level. Glycation is the addition of reducing sugars and related compounds to proteins, DNA and lipids. Glycation theory of aging suggests that modification of proteins by glucose and associated browning (Maillard reaction) leads to the gradual cross-linking, polymerization and development of "Advanced Glycosylation End products" (AGEs) which ultimately lead to the deterioration in structure and function of macromolecules in the body, in a manner similar to the mechanism of pathogenesis of diabetic complications which is described as accelerated aging MATERIALS AND METHODS: Different concentrations of purified calf thymus DNA was incubated with D-glucose for varying time periods. The reaction product was characterised by UV & fluorescence spectroscopy and agarose gel electrophoresis. The heavily glycated-DNA adduct was then used to evaluate anti-glycated-DNA autoantibodies in the sera of healthy individuals of different age groups by direct and inhibition enzyme immunoassay on solid phase. RESULT: DNA hyperchromicity (damage) was proportionate to concentration and incubation period. An increasing association of the glycated-DNA was found with increase in the age and strongest in the eldest age group. CONCLUSION: Glycation of DNA occurring under normal physiological conditions followed by accumulation of modified nucleotides might be an important causative theory explaining aging. KEYWORDS: Glycation, AGEs, Glycated-DNA, Diabetes-accelerated aging
ISSN:0970-1915