Novel Accurate LC-MS/MS Method for Quantitative Determination of Z-lumirubin

Phototherapy (PT) represents a standard treatment for neonatal jaundice. However, no validated clinical method for determination of bilirubin photo oxidation products is available. Thus, the aim of our study was to establish such method for clinical use. A LC-MS/MS assay for simultaneous determinati...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S193
Main Authors: Vitek, L, Jasprova, J, Dvorak, A, Vecka, M, Lenicek, M, Lacina, O, Valaskova, P, Zapadlo, M, Plavka, R, Klan, P
Format: Article
Language:English
Subjects:
Bilirubin
Methods
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Summary:Phototherapy (PT) represents a standard treatment for neonatal jaundice. However, no validated clinical method for determination of bilirubin photo oxidation products is available. Thus, the aim of our study was to establish such method for clinical use. A LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR, the major bilirubin photo oxidation product) and unconjugated bilirubin (UCB) was conducted, using mesobilirubin as an internal standard. LR was prepared by photo-irradiation of UCB and purified by TLC. The assay was tested on human sera from neonates treated with standard PT. Samples were separated on HPLC system with a Poroshell 120 EC-C18 column using a binary mobile phase (NH4F in water/methanol) and the analytes were detected in triple quadrupole mass spectrometer operating in a positive SRM mode. The method was linear up to 100 and 400 pmol/L for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical chemistry. Exposure of newborns to PT raised serum LR concentrations three-fold (p
ISSN:0970-1915