Characterization of Oral ICandida/I spp. Biofilms in Children and Adults Carriers from Eastern Europe and South America

Background: Candida albicans and non-Candida albicans Candida species (NCACs) are known to colonize and invade various tissues, including the oral mucosa. In this work, we aimed to characterize mature biofilms of several Candida spp. clinical isolates (n = 33) obtained from the oral mucosa of childr...

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Published in:Antibiotics (Basel) 2023-04, Vol.12 (5)
Main Authors: Alves, Anelise Maria Costa Vasconcelos, Lopes, Beatriz Oliveira, Leite, Ana Caroline Rocha de Melo, Cruz, Gabriela Silva, Brito, Érika Helena Salles de, Lima, Laritza Ferreira de, Černáková, Lucia, Azevedo, Nuno Filipe, Rodrigues, Célia Fortuna
Format: Article
Language:English
Subjects:
Anidulafungin
Children
Drug resistance in microorganisms
Health aspects
Proteins
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Summary:Background: Candida albicans and non-Candida albicans Candida species (NCACs) are known to colonize and invade various tissues, including the oral mucosa. In this work, we aimed to characterize mature biofilms of several Candida spp. clinical isolates (n = 33) obtained from the oral mucosa of children, adults, and elders of Eastern Europe and South America. Methods: Each strain was evaluated for its capacity to form biofilms in terms of total biomass using the crystal violet assay and for matrix components production (proteins and carbohydrates) using the BCA and phenol-sulfuric tests, respectively. The effect of different antifungals on biofilm formation was studied. Results: in the children's group, a predominance of C. krusei (81%) was observed, while, among adults, the main species was C. albicans (59%). Most strains showed a reduced response to antimicrobial drugs when in biofilm form (p < 0.01). Moreover, it was observed that strains isolated from children produced more matrix, with higher levels of protein and polysaccharides. Conclusions: children were more likely to be infected by NCACs than adults. More importantly, these NCACs were able to form biofilms richer in matrix components. This finding is of clinical importance, particularly in pediatric care, since stronger biofilms are highly associated with antimicrobial resistance, recurrent infections, and higher therapeutic failure.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics12050797