The Potential of ILactiplantibacillus plantarum/I ATCC 14917 in the Development of Alginate-Based Gel Formulations with Anti–IStaphylococcus aureus/I Properties

This study aimed to evaluate the potential of lactic acid bacteria (LAB) in developing alginate-based gel formulations to inhibit Staphylococcus aureus. Initially, the antagonistic actions of three lactic acid bacteria (LAB) (Lacticaseibacillus rhamnosus ATCC 10863, Lactiplantibacillus plantarum ATC...

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Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2023-08, Vol.16 (8)
Main Authors: Sodré, Monaliza Teresa Campos, Ferraz, Fernanda Avelino, Alencar, Amanda Karoline Vieira, Silva, Karinny Farias, Silva, Douglas Henrique dos Santos, Silva, Lucas dos Santos, Araújo Carneiro, Jéssica Silva dos Santos, Monteiro, Cristina Andrade, Silva, Luis Cláudio Nascimento, Monteiro, Andrea de Souza
Format: Article
Language:English
Subjects:
Care and treatment
Gels (Pharmacy)
Health aspects
Materials
Probiotics
Staphylococcus aureus infections
Testing
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Summary:This study aimed to evaluate the potential of lactic acid bacteria (LAB) in developing alginate-based gel formulations to inhibit Staphylococcus aureus. Initially, the antagonistic actions of three lactic acid bacteria (LAB) (Lacticaseibacillus rhamnosus ATCC 10863, Lactiplantibacillus plantarum ATCC 14917, Limosilactobacillus fermentum ATCC 23271) were evaluated against S. aureus ATCC 25923. All tested LAB inhibited S. aureus, but the highest activity was observed for L. plantarum ATCC 14917 (p < 0.05). The antimicrobial effects of L. plantarum ATCC 14917 cell suspensions, sonicate cells extract, and cell-free supernatants (pH 5 or 7) were analyzed using a broth-based assay. The cell suspensions inhibited S. aureus at concentrations ≥ 10%, and these effects were confirmed by a time-kill assay. Alginate-based gels were formulated with cell suspensions, sonicate cells extract, and cell-free supernatant (pH 5). These formulations inhibited S. aureus growth. Based on the results, the alginate gel with cell suspensions at 10% was selected for further characterization. L. plantarum ATCC 14917 survived in the alginate-based gel, especially when stored at 5 °C. At this temperature, the L. plantarum-containing alginate gel was stable, and it was in compliance with microbiological standards. These findings suggest it can be a promising agent for the topical treatment of infections induced by S. aureus.
ISSN:1424-8247
1424-8247
DOI:10.3390/ph16081112