Cytogenomic Characterization of Transposable Elements and Satellite DNA in IPassiflora/I L. Species

The species Passiflora alata, P. cincinnata, and P. edulis have great economic value due to the use of their fruits for human consumption. In this study, we compared the repetitive genome fractions of these three species. The compositions of the repetitive DNA of these three species’ genomes were an...

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Bibliographic Details
Published in:Genes 2024-04, Vol.15 (4)
Main Authors: Silva, Gonçalo Santos, Souza, Margarete Magalhães, Pamponét, Vanessa de Carvalho Cayres, Micheli, Fabienne, Melo, Cláusio Antônio Ferreira de, Oliveira, Sárah Gomes de, Costa, Eduardo Almeida
Format: Article
Language:English
Subjects:
Analysis
Genomes
Genomics
Pharmaceutical industry
Satellite DNA
Transposons
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Summary:The species Passiflora alata, P. cincinnata, and P. edulis have great economic value due to the use of their fruits for human consumption. In this study, we compared the repetitive genome fractions of these three species. The compositions of the repetitive DNA of these three species’ genomes were analyzed using clustering and identification of the repetitive sequences with RepeatExplorer. It was found that repetitive DNA content represents 74.70%, 66.86%, and 62.24% of the genome of P. alata, P. edulis, and P. cincinnata, respectively. LTR Ty3/Gypsy retrotransposons represent the highest genome proportions in P. alata and P. edulis, while Ty1/Copia comprises the largest proportion of P. cincinnata genome. Chromosomal mapping by Fluorescent In Situ Hybridization (FISH) showed that LTR retrotransposons have a dispersed distribution along chromosomes. The subtelomeric region of chromosomes is where 145 bp satellite DNA is located, suggesting that these elements may play important roles in genome structure and organization in these species. In this work, we obtained the first global characterization of the composition of repetitive DNA in Passiflora, showing that an increase in genome size is related to an increase in repetitive DNA, which represents an important evolutionary route for these species.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes15040418