Release of glutamate by the embryonic spinal motoneurons of rat positively regulated by acetylcholine through the nicotinic and muscarinic receptors

• Published in: Neurochemistry international 2006-11, Vol.49 (6), p.584-592
• Main Authors: Barthélémy-Requin, M., Bévengut, M., Portalier, P., Ternaux, J.P.
• Format: Article
• Language: English
• Subjects: Acetylcholine; Acetylcholine - pharmacology; Acetylcholine - physiology; Animals; Biochemistry; Biochemistry, Molecular Biology; Biological and medical sciences; Bioluminescence; Buffers; Calcium; Calcium - pharmacology; Cholinergic receptors; Excitatory Amino Acid Transporter 3; Excitatory Amino Acid Transporter 3 - genetics; Female; Fundamental and applied biological sciences. Psychology; Glutamate release; Glutamic Acid; Glutamic Acid - metabolism; Immunohistochemistry; Life Sciences; Motor Neurons; Motor Neurons - metabolism; Piperidines; Piperidines - pharmacology; Pregnancy; Rats; Rats, Wistar; Receptors, Muscarinic; Receptors, Muscarinic - drug effects; Receptors, Muscarinic - physiology; Receptors, Nicotinic; Receptors, Nicotinic - drug effects; Receptors, Nicotinic - physiology; Spinal Cord; Spinal Cord - cytology; Spinal Cord - embryology; Spinal Cord - growth & development; Vertebrates: nervous system and sense organs
• ISSN: 0197-0186; 1872-9754; 0197-0186
• DOI: 10.1016/j.neuint.2006.04.016

It has been shown that mature neurons in adult vertebrates can co-express glutamate and acetylcholine. Furthermore, interactions at the synaptic level have been demonstrated. In a previous study we found that also motoneurons at early embryonic stages, thus well prior to synapse formation, release acetylcholine, and that glutamate increases this release. We now report the existence of a glutamate release from embryonic motoneurons and the increase of glutamate release by acetylcholine. This effect is mediated by nicotinic and muscarinic cholinergic receptors present on embryonic motoneurons. Using conditions of partial or total depletion of calcium, we show that the glutamate release has two components: one is calcium-dependent and the other calcium-independent. Furthermore, we show that extracellular glutamate can be taken up by motoneurons, probably via the neuronal glutamate transporter EAAC1, which we find to be expressed at this stage. Monitoring of the glutamate release kinetics showed that extracellular glutamate concentration reached a steady-state level, strongly suggesting the establishment of equilibrium between glutamate release and uptake. Altogether, these results support the idea that glutamate can act as a neurotransmitter in embryonic motoneurons. We hypothesise that, glutamate acts as a regulator of motoneuron maturation and spinal cord development.