Peptide fragments released from Phe-caseinomacropeptide in vivo in the rat

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2002-10, Vol.23 (10), p.1773-1781
• Main Authors: Fosset, S, Fromentin, G, Gietzen, D.W, Dubarry, M, Huneau, J.F, Antoine, J.M, Lang, V, Mathieu-Casseron, F, Tomé, D
• Format: Article
• Language: English
• Subjects: Absorption; Administration, Oral; Animal Feed; Animals; Caseinomacropeptide; Caseins - administration & dosage; Caseins - blood; Caseins - metabolism; Caseins - pharmacokinetics; Cattle; Cytoplasmic Vesicles - chemistry; Digestion; domain_sdv.ida.ing; domain_sdv.ida.ing.ing; Food engineering; Glutens; Hydrolysis; Intestinal Absorption; Life Sciences; Male; Pancreas - enzymology; Peptide digestion; Peptide Fragments - administration & dosage; Peptide Fragments - blood; Peptide Fragments - metabolism; Peptide Fragments - pharmacokinetics; Pharmacokinetics; Phe-caseinomacropeptide; Phenylalanine - metabolism; Rat; Rats; Rats, Wistar
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/S0196-9781(02)00134-1

The aim of this study was to investigate the pharmacokinetics of bovine Phe-caseinomacropeptide (Phe-CMP) in the rat after oral administration. This polypeptide was monophosphorylated and mainly nonglycosylated: Phe-CMP-1P. During gastrointestinal digestion and absorption, Phe-CMP-1P was degraded. Intact Phe-CMP-1P and CMP-1P were rapidly released from the stomach. In contrast, partial hydrolysis by pancreatic enzymes was observed. In vitro hydrolysis by brush-border membrane vesicles also indicated that the peptide was degraded. In the blood, “CMP-immunoreactive material” appeared rapidly, reaching a maximum level of 5.5 μg/ml at 60 min.