Using Temperature To Crystallize Proteins: A Mini-Review

The aim of this overview of the work of Marseille Nanoscience Center is to provide biocrystallographs that intend to tackle crystallization with practical examples of the effect of temperature on protein phase separation and crystallization. Crystallization involves two separate processes: nucleatio...

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Bibliographic Details
Published in:Crystal growth & design 2008-12, Vol.8 (12), p.4215-4219
Main Authors: Astier, Jean-Pierre, Veesler, Stéphane
Format: Article
Language:English
Subjects:
Condensed matter: structure, mechanical and thermal properties
Equations of state, phase equilibria, and phase transitions
Exact sciences and technology
General studies of phase transitions
Nucleation
Physics
Solid-solid transitions
Solubility, segregation, and mixing
phase separation
Specific phase transitions
Structure of solids and liquids
crystallography
Structure of specific crystalline solids
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Summary:The aim of this overview of the work of Marseille Nanoscience Center is to provide biocrystallographs that intend to tackle crystallization with practical examples of the effect of temperature on protein phase separation and crystallization. Crystallization involves two separate processes: nucleation and growth, which are rarely completely unconnected. Here we give four concrete examples illustrating how temperature can be used to crystallize proteins. We describe the nucleation of a new phase, solid or liquid and the growth and transformation of existing crystals obtained by seeding or primary or secondary nucleation. The advantages of temperature as a crystallization parameter are constant composition, ease of control and monitoring and reversibility.
ISSN:1528-7483
1528-7505
DOI:10.1021/cg800665b