Identification of surface proteins involved in the adhesion of a probiotic Bacillus cereus strain to mucin and fibronectin

• Published in: Microbiology (Society for General Microbiology) 2009-05, Vol.155 (5), p.1708-1716
• Main Authors: Sanchez, B, Arias, S, Chaignepain, S, Denayrolles, M, Schmitter, J. M, Bressollier, P, Urdaci, M. C
• Format: Article
• Language: English
• Subjects: Bacillus; Bacillus cereus; Bacillus cereus - chemistry; Bacillus cereus - growth & development; Bacillus cereus - physiology; Bacterial Adhesion; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Caco-2 Cells; Chemical Sciences; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; Human health and pathology; Humans; Hépatology and Gastroenterology; Intestinal Mucosa - metabolism; Intestinal Mucosa - microbiology; Life Sciences; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Microbiology; Miscellaneous; Molecular Sequence Data; Mucins - metabolism; Organic chemistry; Probiotics - metabolism; Protein Binding; Spores, Bacterial - growth & development; Spores, Bacterial - physiology
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/mic.0.025288-0

1 UMR 5248 CBMN CNRS-Université Bordeaux 1-ENITAB, Laboratoire de Microbiologie et Biochimie Appliquée, 1 cours du Général de Gaulle, 33175 Gradignan Cedex, France 2 UMR 5248 CBMN CNRS-Université Bordeaux 1-ENITAB, Institut Européen de Chimie et Biologie, B8 avenue des facultés, F-33402 Talence Cedex, France 3 Laboratoire de Chimie des Substances Naturelles, EA 1069, FST, 123 Av. A. Thomas, 87060 Limoges, France Several Bacillus strains isolated from commercial probiotic preparations were identified at the species level, and their adhesion capabilities to three different model intestinal surfaces (mucin, Matrigel and Caco-2 cells) were assessed. In general, adhesion of spores was higher than that of vegetative cells to the three matrices, and overall strain Bacillus cereus CH displayed the best adhesion. Different biochemical treatments revealed that surface proteins of B. cereus CH were involved in the adhesion properties of the strain. Surface-associated proteins from vegetative cells and spores of B. cereus CH were extracted and identified, and some proteins such as S-layer components, flagellin and cell-bound proteases were found to bind to mucin or fibronectin. These facts suggest that those proteins might play important roles in the interaction of this probiotic Bacillus strain within the human gastrointestinal tract. Correspondence M. Urdaci m-urdaci{at}enitab.fr Abbreviations: GIT, gastrointestinal tract These authors contributed equally to this work.