Spatio-temporal diversity of free-living and particle-attached prokaryotes in the tropical lagoon of Ahe atoll (Tuamotu Archipelago) and its surrounding oceanic waters

• Published in: Marine pollution bulletin 2012-01, Vol.65 (10-12), p.525-537
• Main Authors: Michotey, V., Guasco, S., Boeuf, D., Morezzi, N., Durieux, B., Charpy, L., Bonin, P.
• Format: Article
• Language: English
• Subjects: Archaea; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Biodiversity; Biodiversity and Ecology; Coral Reefs; corals; Cyanobacteria; denaturing gradient gel electrophoresis; dry season; Environmental Monitoring; Environmental Sciences; Euryarchaeota; farming systems; Flavobacteria; Genetic Variation; Lagoon; Marine; Ocean, Atmosphere; oysters; Pacific Ocean; Phylogeny; Polynesia; prokaryotic cells; Proteobacteria; quantitative polymerase chain reaction; Sciences of the Universe; Seawater - microbiology; water pollution; wet season
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2012.01.009

► Bacterial density was higher in lagoon with a seasonal tendency. ► Cyanobacteria, to heterotrophic groups in Proteobacteria, and Flavobacteria. ► Euryarchaeota detected with seasonal effect and particle-prevalence for abundance. ► Difference in Lagoon and ocean archaeal community (within MG-II, MBG, Halobacteria). ► Prevalence of members usually associated with coral during rainy season. Spatio-temporal variability of prokaryotic water column communities inside and outside a Polynesian tropical lagoon subjected to pearl oysters farming was assessed in terms of abundance by quantitative PCR and diversity by DGGE. Communities and operational taxonomic units (OTUs) were analysed according to dry/rainy seasons and free-living/particle-attached state. Bacterial density was higher in the lagoon compared to ocean and a seasonal trend was observed. No influence of the localisation within lagoon or of the planktonic/attached states was noticed on bacterial abundance and diversity. The OTUs belonged to Cyanobacteria, to heterotrophic groups in Proteobacteria and Flavobacteria. Archaeal abundance showed seasonal tendency and particle-prevalence, but no effect of lagoon or oceanic location was observed. Lagoon and oceanic archaeal diversity were different and Euryarchaeota (MG-II, MBG, and Halobacteria) were detected. During the dry season, planktonic and particle-associated community differed, whereas at rainy season, both communities were similar and included members usually associated with coral.