Effects of uranium on the metabolism of zebrafish, Danio rerio

• Published in: Aquatic toxicology 2012-08, Vol.118-119, p.9-26
• Main Authors: Augustine, Starrlight, Gagnaire, Béatrice, Adam-Guillermin, Christelle, Kooijman, Sebastiaan A.L.M.
• Format: Article
• Language: English
• Subjects: Animals; chronic exposure; Danio rerio; developmental stages; Dynamic Energy Budget theory; Ecology, environment; energy; environmental exposure; Female; fish; foods; Freshwater; Growth; health hazards; Kinetics; Life Cycle Stages; Life Sciences; Male; Metabolism; Models, Biological; nuclear power; Regression Analysis; Reproduction; Reproduction - drug effects; spawning; Survival Analysis; toxicity; Uranium; Uranium - toxicity; Water Pollutants, Radioactive - toxicity; Zebrafish; Zebrafish - metabolism
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2012.02.029

► Concrete case study of effects of energetics on toxico-kinetics. ► Detecting/quantifying eco-physiological deviations from baseline metabolism using Dynamic Energy Budget theory. ► Quantitative insight on cumulative reproductive investment in zebrafish. ► Integration of spawning behaviour in a mechanistic bioenergetic model. ► Reproductive toxicology of water-borne depleted uranium on zebrafish. ► Assessing organism level effects over its entire life-cycle. The increasing demand for nuclear energy results in heightened levels of uranium (U) in aquatic systems which present a potential health hazard to resident organisms. The aim of this study was to mechanistically assess how chronic exposure to environmentally relevant concentrations of U perturbs the complex interplay between feeding, growth, maintenance, maturation and reproduction throughout the life-cycle of an individual. To this end we analysed literature-based and original zebrafish toxicity data within a same mass and energy balancing conceptual framework. U was found to increase somatic maintenance leading to inhibition of spawning as well as increase hazard rate and costs for growth during the early life stages. The fish's initial conditions and elimination through reproduction greatly affected toxico-kinetics and effects. We demonstrate that growth and reproduction should be measured on specific individuals since mean values were hardly interpretable. The mean food level differed between experiments, conditions and individuals. This last ‘detail’ contributed substantially to the observed variability by its combined effect on metabolism, toxic effects and toxico-kinetics. The significance of this work is that we address exactly how these issues are related and derive conclusions which are independent of experimental protocol and coherent with a very large body of literature on zebrafish eco-physiology.