Blood-feeding and immunogenic Aedes aegypti saliva proteins

Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors i...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2010-05, Vol.10 (10), p.1906-1916
Main Authors: Wasinpiyamongkol, Ladawan, Patramool, Sirilaksana, Luplertlop, Natthanej, Surasombatpattana, Pornapat, Doucoure, Souleymane, Mouchet, François, Séveno, Martial, Remoue, Franck, Demettre, Edith, Brizard, Jean-Paul, Jouin, Patrick, Biron, David G, Thomas, Frédéric, Missé, Dorothée
Format: Article
Language:English
Subjects:
Aades
Aedes
Aedes - chemistry
Aedes - immunology
Aedes aegypti
Analytical, structural and metabolic biochemistry
Animal Feed
Animal proteomics
Animals
Arboviroses
Biodiversity and Ecology
Biological and medical sciences
Blood
Blood meal
Dengue
Dengue fevers
Electrophoresis, Gel, Two-Dimensional
Environmental Sciences
Female
Fundamental and applied biological sciences. Psychology
Human viral diseases
Infectious diseases
Insect Proteins
Insect Proteins - analysis
Insect Proteins - immunology
Medical sciences
Midgut
Miscellaneous
Proteins
Salivary Glands
Salivary Glands - chemistry
Salivary Glands - immunology
Salivary Proteins and Peptides
Salivary Proteins and Peptides - analysis
Salivary Proteins and Peptides - immunology
Tropical viral diseases
Viral diseases
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Summary:Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.200900626