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Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics
We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf,...
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| Published in: | Journal of photochemistry and photobiology. A, Chemistry. Chemistry., 2006-06, Vol.180 (3), p.300-306 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c352t-843dfdfa9f6cd605ea9be96f496300b33ef4388eb1c08bbc31aafb23f5001d053 |
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| cites | cdi_FETCH-LOGICAL-c352t-843dfdfa9f6cd605ea9be96f496300b33ef4388eb1c08bbc31aafb23f5001d053 |
| container_end_page | 306 |
| container_issue | 3 |
| container_start_page | 300 |
| container_title | Journal of photochemistry and photobiology. A, Chemistry. |
| container_volume | 180 |
| creator | Courvoisier, François Boutou, Véronique Guyon, Laurent Roth, Matthias Rabitz, Herschel Wolf, Jean-Pierre |
| description | We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270
nm-pump 810
nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene ( |
| doi_str_mv | 10.1016/j.jphotochem.2006.03.039 |
| format | article |
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nm-pump 810
nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (
Bacillus subtilis,
Escherechia coli and
Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.</description><identifier>ISSN: 1010-6030</identifier><identifier>EISSN: 1873-2666</identifier><identifier>DOI: 10.1016/j.jphotochem.2006.03.039</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Aerosol particles discrimination ; Bio-molecules ultrafast spectroscopy ; Biological intrinsic fluorophore ; Femtosecond fluorescence depletion ; Optics ; Physics ; Real-time particle detection</subject><ispartof>Journal of photochemistry and photobiology. A, Chemistry., 2006-06, Vol.180 (3), p.300-306</ispartof><rights>2006 Elsevier B.V.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-843dfdfa9f6cd605ea9be96f496300b33ef4388eb1c08bbc31aafb23f5001d053</citedby><cites>FETCH-LOGICAL-c352t-843dfdfa9f6cd605ea9be96f496300b33ef4388eb1c08bbc31aafb23f5001d053</cites><orcidid>0000-0002-9715-7404 ; 0000-0002-4767-5940</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://hal.science/hal-00960238$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Courvoisier, François</creatorcontrib><creatorcontrib>Boutou, Véronique</creatorcontrib><creatorcontrib>Guyon, Laurent</creatorcontrib><creatorcontrib>Roth, Matthias</creatorcontrib><creatorcontrib>Rabitz, Herschel</creatorcontrib><creatorcontrib>Wolf, Jean-Pierre</creatorcontrib><title>Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics</title><title>Journal of photochemistry and photobiology. A, Chemistry.</title><description>We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270
nm-pump 810
nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (
Bacillus subtilis,
Escherechia coli and
Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.</description><subject>Aerosol particles discrimination</subject><subject>Bio-molecules ultrafast spectroscopy</subject><subject>Biological intrinsic fluorophore</subject><subject>Femtosecond fluorescence depletion</subject><subject>Optics</subject><subject>Physics</subject><subject>Real-time particle detection</subject><issn>1010-6030</issn><issn>1873-2666</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFUE1LxDAUDKLguvofcvXQ-tK0sT2u68cKC170KCFNX2xK2yxJdmH_vS0rehQG3vCYGZghhDJIGTBx16XdrnXR6RaHNAMQKfAJ1RlZsPKeJ5kQ4nziwCARwOGSXIXQAUCe52xBPh9t0N4OdlTRjl-0Vjqit4oa7wbqYoueqji4sJuY1XSnfLS6x0D3YdYbHKILqN3Y0MH1qPe98rQ5jmqwOlyTC6P6gDc_d0k-np_e15tk-_byul5tE82LLCZlzhvTGFUZoRsBBaqqxkqYvBIcoOYcTc7LEmumoaxrzZlSps64KQBYAwVfkttTbqt6uZvqKH-UTlm5WW3l_AOoBGS8PLBJW5602rsQPJpfAwM5Tyo7-TepnCeVwCdUk_XhZMWpy8Gil0FbHDU21qOOsnH2_5BvybyHjA</recordid><startdate>20060625</startdate><enddate>20060625</enddate><creator>Courvoisier, François</creator><creator>Boutou, Véronique</creator><creator>Guyon, Laurent</creator><creator>Roth, Matthias</creator><creator>Rabitz, Herschel</creator><creator>Wolf, Jean-Pierre</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9715-7404</orcidid><orcidid>https://orcid.org/0000-0002-4767-5940</orcidid></search><sort><creationdate>20060625</creationdate><title>Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics</title><author>Courvoisier, François ; Boutou, Véronique ; Guyon, Laurent ; Roth, Matthias ; Rabitz, Herschel ; Wolf, Jean-Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-843dfdfa9f6cd605ea9be96f496300b33ef4388eb1c08bbc31aafb23f5001d053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aerosol particles discrimination</topic><topic>Bio-molecules ultrafast spectroscopy</topic><topic>Biological intrinsic fluorophore</topic><topic>Femtosecond fluorescence depletion</topic><topic>Optics</topic><topic>Physics</topic><topic>Real-time particle detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Courvoisier, François</creatorcontrib><creatorcontrib>Boutou, Véronique</creatorcontrib><creatorcontrib>Guyon, Laurent</creatorcontrib><creatorcontrib>Roth, Matthias</creatorcontrib><creatorcontrib>Rabitz, Herschel</creatorcontrib><creatorcontrib>Wolf, Jean-Pierre</creatorcontrib><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of photochemistry and photobiology. A, Chemistry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Courvoisier, François</au><au>Boutou, Véronique</au><au>Guyon, Laurent</au><au>Roth, Matthias</au><au>Rabitz, Herschel</au><au>Wolf, Jean-Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics</atitle><jtitle>Journal of photochemistry and photobiology. A, Chemistry.</jtitle><date>2006-06-25</date><risdate>2006</risdate><volume>180</volume><issue>3</issue><spage>300</spage><epage>306</epage><pages>300-306</pages><issn>1010-6030</issn><eissn>1873-2666</eissn><abstract>We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270
nm-pump 810
nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (<2%), despite almost identical absorption/emission spectra. We demonstrate that this process in Trp is so robust that it still occurs in living bacteria (
Bacillus subtilis,
Escherechia coli and
Enterococcus fæcalis) but is absent for pure diesel fuel. This remarkable difference between biological and organic aerosols can be exploited to discriminate among them.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.jphotochem.2006.03.039</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-9715-7404</orcidid><orcidid>https://orcid.org/0000-0002-4767-5940</orcidid></addata></record> |
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| ispartof | Journal of photochemistry and photobiology. A, Chemistry., 2006-06, Vol.180 (3), p.300-306 |
| issn | 1010-6030 1873-2666 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Aerosol particles discrimination Bio-molecules ultrafast spectroscopy Biological intrinsic fluorophore Femtosecond fluorescence depletion Optics Physics Real-time particle detection |
| title | Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics |
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