Conformational Manipulation of DNA in Nanochannels Using Hydrodynamics

The control over DNA elongation in nanofluidic devices holds great potential for large-scale genomic analysis. So far, the manipulation of DNA in nanochannels has been mostly carried out with electrophoresis and seldom with hydrodynamics, although the physics of soft matter in nanoscale flows has ra...

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Bibliographic Details
Published in:Macromolecules 2013-08, Vol.46 (15), p.6195-6202
Main Authors: He, Qihao, Ranchon, Hubert, Carrivain, Pascal, Viero, Yannick, Lacroix, Joris, Blatché, Charline, Daran, Emmanuelle, Victor, Jean-Marc, Bancaud, Aurélien
Format: Article
Language:English
Subjects:
Applied sciences
Biological Physics
Exact sciences and technology
Miscellaneous
Natural polymers
Physicochemistry of polymers
Physics
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Summary:The control over DNA elongation in nanofluidic devices holds great potential for large-scale genomic analysis. So far, the manipulation of DNA in nanochannels has been mostly carried out with electrophoresis and seldom with hydrodynamics, although the physics of soft matter in nanoscale flows has raised considerable interest over the past decade. In this report the migration of DNA is studied in nanochannels of lateral dimension spanning 100 to 500 nm using both actuation principles. We show that the relaxation kinetics are 3-fold slowed down and the extension increases up to 3-fold using hydrodynamics. We propose a model to account for the onset in elongation with the flow, which assumes that DNA response is determined by the shear-driven lift forces mediated by the proximity of the channels’ walls. Overall, we suggest that hydrodynamic actuation allows for an improved manipulation of DNA in nanochannels.
ISSN:0024-9297
1520-5835
DOI:10.1021/ma400575h